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Rabbit IL-1β(Interleukin 1 Beta) ELISA Kit

  • Cat.No.:E-EL-RB0013

  • Reactivity: Rabbit

To Purchase E-EL-RB0013

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $588

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rabbit IL-1β in samples. No significant cross-reactivity or interference between Rabbit IL-1β and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rabbit IL-1β concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IL-1β. You can calculate the concentration of Rabbit IL-1β in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rabbit IL-1β were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rabbit IL-1β were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 47.59 121.44 462.88 47.73 124.59 497.69
Standard deviation 2.65 7.16 14.86 3.08 6.28 23.64
CV (%) 5.57 5.90 3.21 6.45 5.04 4.75


The recovery of Rabbit IL-1β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-100 92
EDTA plasma (n=8) 94-104 99
Cell culture media (n=8) 91-101 96


Samples were spiked with high concentrations of Rabbit IL-1β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P14628
SynonymsIL1B, IL1-BETA, IL1F2, catabolin
Research AreaCancer, Cardiovascular, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. EBioMedicine (2018) IF: 6.183
    Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7

    DOI: 10.1016/j.ebiom.2018.09.020

    PMID: 30243490

    Sample: Plasma
  2. Toxins (2018) IF: 3.273
    Establishment of a New Zealand White Rabbit Model for Lethal Toxin (LT) Challenge and Efficacy of Monoclonal Antibody 5E11 in the LT-Challenged Rabbit Model

    DOI: 10.3390/toxins10070289

    Sample: Serum
  3. World Journal of Stem Cells (2019) IF: 3.534
    Anti-osteoarthritis effect of a combination treatment with human adipose tissue-derived mesenchymal stem cells and thrombospondin 2 in rabbits

    DOI: 10.4252/wjsc.v11.i12.1115

    PMID: 31875872

    Sample: Synovia
  4. Annals of Translational Medicine (2022) IF: 3.616
    Seawater immersion exacerbates the pathological changes caused by incisive corneal injury in rabbit eyes

    DOI: 10.21037/atm-22-2198

    Sample: aqueous humor
  5. Lipids in Health and Disease (2021) IF: 3.876
    CRISPR/Cas9-mediated knockout of APOC3 stabilizes plasma lipids and inhibits atherosclerosis in rabbits

    DOI: 10.1186/s12944-021-01605-7

    PMID: 34922545

    Sample: plasma
    Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1–7) upregulation

    DOI: 10.1016/j.biopha.2017.11.151

    Sample: Cell culture supernatant,Serum
  7. CURRENT EYE RESEARCH (2019) IF: 1.672
    The Efficiency of Cyclosporine A-Eluting Contact Lenses for the Treatment of Dry Eye

    DOI: 10.1080/02713683.2018.1563702

    PMID: 30580651

    Sample: Tissue homogenate
  8. Molecular Medicine Reports (2019) IF: 1.851
    Acupuncture attenuates renal interstitial fibrosis via the TGF‑β/Smad pathway

    DOI: 10.3892/mmr.2019.10470

    Sample: blood
  9. Molecular Medicine Reports (2018) IF: 1.922
    Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits

    DOI: 10.3892/mmr.2017.8304

    Sample: joint fluid
  10. THERIOGENOLOGY (2017) IF: 1.986
    Effects of local lipopolysaccharide administration on the expression of Toll-like receptor 4 and pro-inflammatory cytokines in uterus and oviduct of rabbit does

    DOI: 10.1016/j.theriogenology.2017.10.046

    Sample: Plasma
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