Current Position:Home>>Kits>>ELISA Kits>> Rat HDL(High Density Lipoprotein) ELISA Kit

Rat HDL(High Density Lipoprotein) ELISA Kit

Cat.No.:E-EL-R0504
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R0504

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	3.13-200 ng/mL
Sensitivity	1.88 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat HDL in samples. No significant cross-reactivity or interference between Rat HDL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat HDL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HDL. You can calculate the concentration of Rat HDL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HDL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HDL were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	9.72	19.10	68.27	9.69	17.42	70.91
Standard deviation	0.65	0.86	2.38	0.59	0.97	3.79
CV (%)	6.69	4.50	3.49	6.09	5.57	5.34

Recovery

The recovery of Rat HDL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-99	91
EDTA plasma (n=8)	88-99	94
Cell culture media (n=8)	85-98	91

Linearity

Samples were spiked with high concentrations of Rat HDL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Cardiovascular, Signal Transduction, Cancer, Metabolism

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

International Journal of Nanomedicine (2017) IF: 4.3
Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo

DOI: 10.2147/IJN.S134897

Sample: Cell culture supernatant,Serum
INFLAMMOPHARMACOLOGY (2020) IF: 3.238
Perindopril ameliorates experimental Alzheimer’s disease progression: role of amyloid β degradation, central estrogen receptor and hyperlipidemic-lipid raft signaling

DOI: 10.1007/s10787-020-00724-4

Sample: Serum,Tissue homogenate
JOURNAL OF PHARMACY AND PHARMACOLOGY (2021) IF: 3.765
Cissus quadrangularis extract attenuates diabetic nephropathy by altering SIRT1/DNMT1 axis

DOI: 10.1093/jpp/rgab078

PMID: 34128987
Sample: plasma
International Journal of Peptide Research and Therapeutics (2022) IF: 2.191
Fibroblast Growth Factor 21 Ameliorates Endothelin I-Induced Hypertension Partly Through PPAR γ Pathway

DOI: 10.1007/s10989-022-10408-y

Sample: serum
SOUTH AFRICAN JOURNAL OF BOTANY (2021) IF: 2.315
Investigation of therapeutic potential of three endemic Cirsium species for global health problem obesity

DOI: 10.1016/j.sajb.2021.05.004

Sample: Serum
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES (2022) IF: 1.885
Effect of oligosaccharides on the antioxidant, lipid and inflammatory profiles of rats with streptozotocin-induced diabetes mellitus

DOI: 10.1515/znc-2021-0215

Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha