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Cat.No.:E-EL-R0500
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 78.13-5000 pg/mL |
Sensitivity | 46.88 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat Hepc in samples. No significant cross-reactivity or interference between Rat Hepc and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat Hepc concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
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Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat Hepc were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat Hepc were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 254.50 | 612.40 | 2443.00 | 232.20 | 578.00 | 2321.70 |
Standard deviation | 13.50 | 29.40 | 80.60 | 14.20 | 31.20 | 90.50 |
CV (%) | 5.30 | 4.80 | 3.30 | 6.12 | 5.40 | 3.90 |
The recovery of Rat Hepc spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 90-100 | 95 |
EDTA plasma (n=8) | 92-104 | 97 |
Cell culture media (n=8) | 91-105 | 97 |
Samples were spiked with high concentrations of Rat Hepc and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Hepcidin Antimicrobial Peptide |
Research Area | Cancer, Cardiovascular, Metabolism, Signal transduction |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
DOI: 10.1039/D2FO02226E |
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Sample: serum |
PMID: 35184371 |
Sample: serum |
PMID: 31499193 |
Sample: Serum,Tissue homogenate |
Sample: Plasma |
DOI: 10.1155/2022/3972829 |
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Sample: H9C2 cell |