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Rat IL-17A(Interleukin 17A) ELISA Kit

Citations(29)Uniprot : Q61453

  • Cat.No.:E-EL-R0566

  • Reactivity: Rat
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-17A in samples. No significant cross-reactivity or interference between Rat IL-17A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-17A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-17A. You can calculate the concentration of Rat IL-17A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-17A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-17A were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 55.20 149.70 486.20 50.50 153.30 468.50
Standard deviation 3.40 7.90 18.00 2.60 8.70 21.60
CV (%) 6.16 5.28 3.70 5.15 5.68 4.61

Recovery

The recovery of Rat IL-17A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-110 101
EDTA plasma (n=8) 97-111 102
Cell culture media (n=8) 93-104 99

Linearity

Samples were spiked with high concentrations of Rat IL-17A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q61453
SynonymsCTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Journal of Crohns & Colitis (2015) IF: 9.071
    Diet-Induced Hyperhomocysteinaemia Increases Intestinal Inflammation in an Animal Model of Colitis

    DOI: 10.1093/ecco-jcc/jjv094
    Sample: Plasma
  2. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Investigation of the impact of rosuvastatin and telmisartan in doxorubicin-induced acute cardiotoxicity

    DOI: 10.1016/j.biopha.2022.113673
    Sample: serum
  3. PHYTOMEDICINE (2022) IF: 6.656
    Intragastric administration of Pien Tze Huang enhanced wound healing in diabetes by inhibiting inflammation and improving energy generation

    DOI: 10.1016/j.phymed.2022.154578
    Sample: skin tissue
  4. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2022) IF: 5.112
    Thermosensitive smart hydrogel of PEITC ameliorates the therapeutic efficacy in rheumatoid arthritis

    DOI: 10.1016/j.ejps.2022.106367
    Sample: serum
  5. Pharmaceuticals (2022) IF: 5.215
    IL-17/Notch1/STAT3 Pathway Contributes to 5-Fluorouracil-Induced Intestinal Mucositis in Rats: Amelioration by Thymol Treatment

    DOI: 10.3390/ph15111412
    Sample: intestinal tissue
  6. PHYTOMEDICINE (2021) IF: 5.34
    Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

    DOI: 10.1016/j.phymed.2021.153875

    PMID: 34911003

    		 Sample: serum
  7. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119
    Platelet-rich plasma exhibits anti-inflammatory effect and attenuates cardiomyocyte damage by reducing NF-κB and enhancing VEGF expression in isoproterenol induced cardiotoxicity model

    DOI: 10.1002/tox.23456

    PMID: 35014750

    		 Sample: cardiac tissue
  8. ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119
    Imbalanced inflammatory response in subchronic arsenic-induced liver injury and the protective effects of Ginkgo biloba extract in rats: Potential role of cytokines mediated cell–cell interactions

    DOI: 10.1002/tox.23324
    Sample: serum,liver
  9. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219
    Neuroprotective efficacy of 4-Hydroxyisoleucine in experimentally induced intracerebral hemorrhage

    DOI: 10.1016/j.sjbs.2021.07.010

    PMID: 34764759

    		 Sample: brain
  10. Scientific Reports (2017) IF: 4.259
    Effect of Qianghuo Erhuang Decoction on T Regulatory and T Helper 17 Cells in Treatment of Adjuvant-induced Arthritis in Rats

    DOI: 10.1038/s41598-017-17566-w
    Sample: Serum
  11. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways

    DOI: 10.1016/j.jep.2021.114791

    PMID: 34737112

    		 Sample: serum
  12. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY (2022) IF: 4.369
    BMSC-EVs overexpressing PD-L1 ameliorate DSS-induced ulcerative colitis in rats by regulating Th17/Treg cell balance through PTEN/PI3K/AKT/mTOR axis

    DOI: 10.1111/jgh.15987
    Sample: blood,colon
  13. EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 4.432
    PSTPIP2 attenuates joint damage and suppresses inflammation in adjuvant-induced arthritis

    DOI: 10.1016/j.ejphar.2019.172558
    Sample: serum
  14. PHYTOTHERAPY RESEARCH (2017) IF: 3.092
    Antiinflammation Effects and Mechanisms Study of Geniposide on Rats with Collagen‐Induced Arthritis

    DOI: 10.1002/ptr.5775
    Sample: Cell culture supernatant
  15. MOLECULES (2018) IF: 3.098
    Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats

    DOI: 10.3390/molecules23010091
    Sample: fibroblast-like synoviocytes (FLS)
  16. Bioengineered (2021) IF: 3.269
    Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes

    DOI: 10.1080/21655979.2021.1999554

    PMID: 34719315

    		 Sample: serum
  17. BMC NEUROSCIENCE (2021) IF: 3.288
    Metformin attenuates sepsis-induced neuronal injury and cognitive impairment

    DOI: 10.1186/s12868-021-00683-8

    PMID: 34911449

    		 Sample: hippocampus tissue
  18. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity

    DOI: 10.1016/j.biopha.2018.07.081
    Sample: Serum
  19. DISEASE MARKERS (2022) IF: 3.464
    Estrogen Protects against Renal Ischemia-Reperfusion Injury by Regulating Th17/Treg Cell Immune Balance

    DOI: 10.1155/2022/7812099

    PMID: 36246554

    		 Sample: serum,kidney tissues
  20. PHARMACEUTICAL BIOLOGY (2021) IF: 3.503
    Guizhi-Shaoyao-Zhimu decoction attenuates bone erosion in rats that have collagen-induced arthritis via modulating NF‐κB signalling to suppress osteoclastogenesis

    DOI: 10.1080/13880209.2021.1876100
    Sample: serum
  21. Frontiers in Pharmacology (2018) IF: 3.831
    Anti-inflammatory Mechanism of Geniposide: Inhibiting the Hyperpermeability of Fibroblast-Like Synoviocytes via the RhoA/p38MAPK/NF-κB/F-Actin Signal Pathway

    DOI: 10.3389/fphar.2018.00105
    Sample: fibroblast-like synoviocytes (FLSs)
  22. Brain and Behavior (2019) IF: 2.072
    Th17 cells and their cytokines serve as potential therapeutic target in experimental autoimmune neuritis

    DOI: 10.1002/brb3.1478

    PMID: 31742934

    		 Sample: Serum,Tissue homogenate
  23. Iranian Journal of Basic Medical Sciences (2020) IF: 2.146
    Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain

    DOI: 10.22038/ijbms.2020.45193.10527

    PMID: 33149866

    		 Sample: Tissue homogenate
  24. Toxicology Research (2020) IF: 2.283
    The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats

    DOI: 10.1093/toxres/tfaa030

    PMID: 32670559

    		 Sample: Tissue homogenate
  25. Metabolic Syndrome and Related Disorders (2022) IF: 2.363
    Naringenin Prevents Inflammation, Apoptosis, and DNA Damage in Potassium Oxonate-Induced Hyperuricemia in Rat Liver Tissue: Roles of Cytochrome C, NF-κB, Caspase-3, and 8-Hydroxydeoxyguanosine

    DOI: 10.1089/met.2022.0028

    PMID: 35796694

    		 Sample: Liver tissue
  26. INTERNATIONAL IMMUNOPHARMACOLOGY (2014) IF: 2.711
    Effects and mechanisms of Geniposide on rats with adjuvant arthritis

    DOI: 10.1016/j.intimp.2014.02.021
    Sample: Cell lysate
  27. DIGESTIVE DISEASES AND SCIENCES (2018) IF: 2.819
    T Cells into Th17 Cells

    DOI: 10.1007/s10620-018-5177-2
    Sample: Cell culture supernatant
  28. VIRUS RESEARCH (2014) IF: 2.827
    Galectin-9 ameliorates respiratory syncytial virus-induced pulmonary immunopathology through regulating the balance between Th17 and regulatory T cells

    DOI: 10.1016/j.virusres.2014.10.011
    Sample: Tissue homogenate
  29. JOURNAL OF CELLULAR BIOCHEMISTRY (2018) IF: 2.959
    Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

    DOI: 10.1002/jcb.28243

    PMID: 30520122

    		 Sample: Serum,Bronchoalveolar Lavage Fluid
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