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Rat IL-17A(Interleukin 17A) ELISA Kit

Citations(11)Uniprot : Q61453

Cat.No.:E-EL-R0566
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat IL-17A in samples. No significant cross-reactivity or interference between Rat IL-17A and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat IL-17A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-17A. You can calculate the concentration of Rat IL-17A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-17A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-17A were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	55.20	149.70	486.20	50.50	153.30	468.50
Standard deviation	3.40	7.90	18.00	2.60	8.70	21.60
CV (%)	6.16	5.28	3.70	5.15	5.68	4.61

Recovery

The recovery of Rat IL-17A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-110	101
EDTA plasma (n=8)	97-111	102
Cell culture media (n=8)	93-104	99

Linearity

Samples were spiked with high concentrations of Rat IL-17A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q61453
Synonyms	CTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Journal of Crohn's & Colitis (2015) IF: 5.813

Diet Induced Hyperhomocysteinemia Increases Intestinal Inflammation In An Animal Model Of Colitis.

DOI: 10.1093/ecco-jcc/jjv094
PMID: 26071411	Sample: Plasma

Scientific reports (2017) IF: 4.259

Effect Of Qianghuo Erhuang Decoction On T Regulatory And T Helper 17 Cells In Treatment Of Adjuvant-Induced Arthritis In Rats.

DOI: 10.1038/s41598-017-17566-w
PMID: 29222448	Sample: Serum

Toxins (2018) IF: 3.273

The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.

DOI: 10.3390/toxins10110449
PMID: 30384460	Sample: Serum, Tissue homogenate

Biomedicine & Pharmacotherapy (2018) IF: 3.457

Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

DOI: 10.1016/j.biopha.2018.07.081
PMID: 30119204	Sample: Serum

Phytotherapy Research (2017) IF: 3.092

Antiinflammation Effects And Mechanisms Study Of Geniposide On Rats With Collagen-Induced Arthritis.

DOI: 10.1002/ptr.5775
PMID: 28127805	Sample: Cell culture supernatant

Toxicology Research (2020) IF: 2.283

The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats

DOI: 10.1093/toxres/tfaa030
PMID: 32670559	Sample: Tissue homogenate

Brain and Behavior (2019) IF: 2.091

Th17 cells and their cytokines serve as potential therapeutic target in experimental autoimmune neuritis

DOI: 10.1002/brb3.1478
PMID: 31742934	Sample: Serum,Tissue homogenate

Journal of cellular biochemistry (2018) IF: 2.959

Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

DOI: 10.1002/jcb.28243
PMID: 30520122	Sample: Serum,Bronchoalveolar Lavage Fluid

Digestive diseases and sciences (2018) IF: 2.819

Effect of Homocysteine on the Differentiation of CD4+ T Cells into Th17 Cells.

DOI: 10.1007/s10620-018-5177-2
PMID: 29974377	Sample: Cell culture supernatant

Virus Research (2014) IF: 2.628

Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.

DOI: 10.1016/j.virusres.2014.10.011
PMID: 25451068	Sample: Tissue homogenate

Int J Recent Sci Res (2015)

Interleukin-17: a cardiac biomarker in estimation of cardioprotective effects of tacrolimus in doxorubicin-induced cardiotoxicity: animal model study


	Sample: Serum

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