Rat INS(Insulin) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-R2466

      Synonyms:IDDM2, ILPR, IRDN, MODY10

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $580

      Reactivity: Rat

      Detection Range: 0.31~20 ng/mL

      Sensitivity: 0.19 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat INS. You can calculate the concentration of Rat INS in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Rat
      Detection Method Colormetric
      Detection Range 0.31—20 ng/mL
      Sensitivity 0.19 ng/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (ng/mL)
      O.D Average Corrected
      20 2.415
      2.451
      2.433 2.38
      10 1.53
      1.588
      1.559 1.506
      5 0.888
      0.87
      0.879 0.826
      2.5 0.433
      0.439
      0.436 0.383
      1.25 0.208
      0.196
      0.202 0.149
      0.63 0.153
      0.143
      0.148 0.095
      0.31 0.097
      0.107
      0.102 0.049
      0 0.047
      0.059
      0.053 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat INS were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat INS were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (ng/mL) 0.99 2.68 6.73 1.00 2.82 6.90
      Standard deviation 0.06 0.14 0.25 0.07 0.16 0.35
      C V (%) 6.06 5.22 3.71 7.00 5.67 5.07

      Recovery

      The recovery of Rat INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 86-101 93
      EDTA plasma (n=5) 93-105 98
      Cell culture media (n=5) 86-98 92

      Linearity

      Samples were spiked with high concentrations of Rat INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 90-103 86-98 96-112
      Average (%) 95 93 102
      1:4 Range (%) 92-108 85-98 90-101
      Average (%) 98 92 95
      1:8 Range (%) 90-104 86-97 84-96
      Average (%) 98 91 89
      1:16 Range (%) 87-101 82-93 86-98
      Average (%) 93 88 92

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

      2.Remove the liquid.

      3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

      4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

      6.Add 50 μL Stop Solution.

      7.Read at 450 nm immediately. Calculation of results.

      Citations

      1. Publication: Li H, Wang Y P, Zhang L N, et al. Perivascular Adipose Tissue-Derived Leptin Promotes Vascular Smooth Muscle Cell Phenotypic Switching Via P38 Mitogen-Activated Protein Kinase In Metabolic Syndrome Rats[J]. Experimental Biology and Medicine, 2014, 239(8): 954-965.
        Sample Type: Blood
      2. Publication: Zhang Y, Imam M U, Ismail M, et al. High Fat Diet-Induced Inflammation And Oxidative Stress Are Attenuated By N-Acetylneuraminic Acid In Rats[J]. Journal of Biomedical Science, 2015.
        Sample Type: Serum
      3. Publication: Liu H, Liu H Y, Jiang Y N, et al. Protective Effect Of Thymoquinone Improves Cardiovascular Function, And Attenuates Oxidative Stress, Inflammation And Apoptosis By Mediating The Pi3K/Akt Pathway In Diabetic Rats[J]. Molecular Medicine Reports, 2016, 13(3): 2836-2842.
        Sample Type: blood
      4. Publication: Hong Y P, Guo W Y, Wang W X, et al. 4-Phenylbutyric Acid Attenuates Pancreatic Beta-Cell Injury In Rats With Experimental Severe Acute Pancreatitis[J].International Journal of Endocrinology, 2016.
        Sample Type: Serum
      5. Publication: Guo Y, Liu C Q, Shan C X, et al. Gut Microbiota After Roux-En-Y Gastric Bypass And Sleeve Gastrectomy In A Diabetic Rat Model: Increased Diversity And Associations Of Discriminant Genera With Metabolic Changes[J]. Diabetes/Metabolism Research and Reviews, 2017.
        Sample Type: Serum
      6. Publication: Shang S W, Yang J L , Huang F, et al. Modified Si-Miao-San Ameliorates Pancreatic B Cell Dysfunction By Inhibition Of Reactive Oxygen Species-Associated Inflammation Through Amp-Kinase Activation[J]. Chinese Journal of Natural Medicines, 2014, 12(5): 351-360.
        Sample Type: culture supernatant
      7. Publication: He J L, Zhao M, Xia J J, et al. FGF21 Ameliorates The Neurocontrol Of Blood Pressure In The High Fructose-Drinking Rats[J]. Scientific Reports, 2016.
        Sample Type: Serum
      8. Publication: Jin H, Ning Y, Zhou H, et al. Il-6 Promotes Islet Β-Cell Dysfunction In Rat Collagen-Induced Arthritis[J]. Journal of Diabetes Research, 2016.
        Sample Type: blood
      9. Publication: Adeyemi W J, Olayaki L A. Effects Of Single Or Combined Induction Of Diabetes Mellitus And Knee Osteoarthritis On Some Biochemical And Haematological Parameters In Rats[J]. Experimental and Molecular Pathology, 2017.
        Sample Type: Serum
      10. Publication: Zarzour A, Hamdan R, Ahmad M, et al. Phyllanthus Niruri Standardized Extract Alleviates The Progression Of Non-Alcoholic Fatty Liver Disease And Decreases Atherosclerotic Risk In Sprague-Dawley Rats[J]. Nutrients, 2017.
        Sample Type: Serum
      11. Publication: Imam M U, Ismail M, Mchinnappan S. Effects Of The Aqueous Extracts Of Rhodamnia Cinerea On Metabolic Indices And Sorbitol-Related Complications In Type 2 Diabetic Rats[J]. Sains Malaysiana, 2017, 46(4): 589-595.
        Sample Type: Serum
      12. Publication: Durak A, Olgar Y, Tuncay E, et al. Onset Of Depressed Heart Work Is Correlated With The Increased Heart Rate And Shorten QT-Interval In High-Carbohydrate Fed Overweight Rats[J]. Canadian Journal of Physiology And Pharmacology, 2017.
        Sample Type: Serum
      13. Publication: Sibiya N, Ngubane P, Mabandla M. The Ameliorative Effect Of Pectin-Insulin Patch On Renal Injury In Streptozotocin-Induced Diabetic Rats[J]. Kidney & Blood Pressure Research, 2017, 42: 530-540.
        Sample Type: Urine, Tissue homogenate, Plasma
      14. Publication: Yu J, Yang J, Luo Y, et al. The Adverse Effects Of Chronic Low-Dose Exposure To Nonylphenol On Type 2 Diabetes Mellitus In High Sucrose-High Fat Diet-Treated Rats[J]. Islets, 2017.
        Sample Type: Plasma
      15. Publication: Lembede B W, Erlwanger K H, Nkomozepi P, et al. Effect Of Neonatal Orally Administered S-allyl Cysteine In High-Fructose Diet Fed Wistar Rats[J]. Journal of Developmental Origins Of Health And Disease, 2017.
        Sample Type: Plasma
      16. Publication: Long G H, Zhang G T, Zhang F T, et al. Cotransplantation Of mesenchymal Stem Cells And Immature Dendritic Cells Potentiates The Blood Glucose Control Of Islet Allografts[J]. BioMed Research International, 2017.
        Sample Type: culture supernatant
      17. Publication: Durak A, Olgar Y, Tuncay E, et al. Onset Of Depressed Heart Work Is Correlated With The Increased Heart Rate And Shortened QT Interval In High-Carbohydrate Fed Overweight Rats[J]. Canadian Journal of Physiology And Pharmacology, 2017, 95(11): 1335-1342.
        Sample Type: Serum
      18. Publication: Zhu S, Chen S, Gao Y, et al. Enhanced Oral Bioavailability Of Insulin Using PLGA Nanoparticles Co-Modified With Cell-Penetrating Peptides And Engrailed Secretion Peptide (Sec)[J]. Drug Delivery, 2016, 23(6): 1980-1991.
        Sample Type: Plasma
      19. Publication: Yida Z, Imam M U, Ismail M, et al. N-Acetylneuraminic Acid Supplementation Prevents High Fat Diet-Induced Insulin Resistance In Rats Through Transcriptional And Nontranscriptional Mechanisms[J]. BioMed Research International, 2015.
        Sample Type: Serum
      20. Publication: Zhang Y D, Imam M U, Ismail M, et al. Edible Bird'S Nest Prevents High Fat Diet-Induced Insulin Resistance In Rats[J]. Journal of Diabetes Research, 2015.
        Sample Type: Serum
      21. Publication: Fan Y, He Z, Wang W, et al. Tangganjian decoction ameliorates type 2 diabetes mellitus and nonalcoholic fatty liver disease in rats by activating the IRS/PI3K/AKT signaling pathway[J]. Biomedicine & Pharmacotherapy, 2018, 106: 733-737.
        Sample Type: Serum
      22. Publication: Muhammad N. Effects of Methanolic Extract of Moringa oleifera leaves on Fructose-Induced Metabolic dysfunction in growing Sprague Dawley Rats[D]. , 2017.
        Sample Type: Serum
      23. Publication: Wahjuningsih S B, Haslina S U, Wijanarka A. Hypoglycemic Effect of Analog Rice Made from Modified Cassava Flour (Mocaf), Arrowroot Flour and Kidney Bean Flour on STZ-NA Induced Diabetic Rats[J]. Nutrition, 2018, 10(1): 8-15.
        Sample Type: Serum
      24. Publication: Wihastuti T A, Heriansyah T, Hanifa H, et al. Darapladib inhibits atherosclerosis development in type 2 diabetes mellitus Sprague-Dawley rat model[J]. Endocrine regulations, 2018, 52(2): 69-75.
        Sample Type: Rat
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