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Rat KISS1(Kisspeptin 1) ELISA Kit

  • Cat.No.:E-EL-R2530

  • Reactivity: Rat

To Purchase E-EL-R2530

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 78.13-5000 pg/mL
Sensitivity 46.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat KISS1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat KISS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat KISS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat KISS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat KISS1. You can calculate the concentration of Rat KISS1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat KISS1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat KISS1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 228.82 578.41 2346.06 244.62 578.38 2521.30
Standard deviation 13.41 32.04 79.30 15.78 27.94 92.28
CV (%) 5.86 5.54 3.38 6.45 4.83 3.66


The recovery of Rat KISS1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-98 92
EDTA plasma (n=8) 90-103 96
Cell culture media (n=8) 87-101 94


Samples were spiked with high concentrations of Rat KISS1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Frontiers in Physiology (2022) IF: 4.755
    Serum kisspeptin levels mainly depend on ovarian expression of Kiss1 mRNA in female rats

    DOI: 10.3389/fphys.2022.998446

    Sample: Serum
  2. PHYSIOLOGY & BEHAVIOR (2021) IF: 3.244
    Chronic irisin exposure decreases sexual incentive motivation in female rats

    DOI: 10.1016/j.physbeh.2021.113341

    PMID: 33508315

    Sample: Serum
  3. TOXICOLOGY LETTERS (2019) IF: 3.499
    Effects of chlorocholine chloride on pubertal development and reproductive functions in male rats

    DOI: 10.1016/j.toxlet.2019.10.024

    PMID: 31689472

    Sample: Serum
  4. ENDOCRINE (2022) IF: 3.633
    Beneficial effects of irisin in experimental paroxetine-induced hyperprolactinemia

    DOI: 10.1007/s12020-022-03010-1

    PMID: 35226247

    Sample: Serum
  5. ENDOCRINE (2022) IF: 3.925
    Acetate restores hypothalamic-adipose kisspeptin status in a rat model of PCOS by suppression of NLRP3 immunoreactivity

    DOI: 10.1007/s12020-022-03191-9

    PMID: 36114434

    Sample: Hypothalamic Tissue,Vat
  6. TOXICOLOGY LETTERS (2021) IF: 4.374
    Effects of prenatal chlorocholine chloride exposure on pubertal development and reproduction of male offspring in rats

    DOI: 10.1016/j.toxlet.2021.08.005

    PMID: 34411681

    Sample: Serum
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