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Rat TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit

  • Cat.No.:E-EL-R1253

  • Reactivity: Rat

To Purchase E-EL-R1253

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Rat TNNI3/cTn-I and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNNI3/cTn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNNI3/cTn-I. You can calculate the concentration of Rat TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TNNI3/cTn-I were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TNNI3/cTn-I were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 102.40 248.30 900.40 97.00 237.90 894.70
Standard deviation 5.70 14.90 36.90 5.10 11.40 37.60
CV (%) 5.57 6.00 4.10 5.26 4.79 4.20

Recovery

The recovery of Rat TNNI3/cTn-I spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-110 101
EDTA plasma (n=8) 93-111 101
Cell culture media (n=8) 93-107 100

Linearity

Samples were spiked with high concentrations of Rat TNNI3/cTn-I and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsTNNC1, cTnI, CMD1FF, CMD2A, CMH7, RCM1

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Acta Pharmacologica Sinica (2020) IF: 5.064
    Exogenous NADPH ameliorates myocardial ischemia-reperfusion injury in rats through activating AMPK/mTOR pathway

    DOI: 10.1038/s41401-019-0301-1

    PMID: 31776448

    Sample: Serum
  2. European review for medical and pharmacological sciences (2020) IF: 3.024
    The effect of myocardial infarction-associated transcript 2 (miR-2) and miR-101 on sepsis-induced myocardial injury in rats

    DOI: 10.26355/eurrev_202006_21528

    PMID: 99999999

    Sample: Serum
  3. Inflammopharmacology (2019) IF: 3.238
    Urotensin-#receptor antagonist SB-706375 protected isolated rat heart from ischaemia-reperfusion injury by attenuating myocardial necrosis via RhoA/ROCK/RIP3 signalling pathway

    DOI: 10.1007/s10787-019-00598-1

    PMID: 31168686

    Sample: Bronchoalveolar Lavage Fluid
  4. Journal of Cellular Biochemistry (2017) IF: 3.085
    Functional And Structural Assessment Of The Effect Of Human Umbilical Cord Blood Mesenchymal Stem Cells In Doxorubicin-Induced Cardiotoxicity.

    DOI: 10.1002/jcb.26168

    PMID: 28543396

    Sample: Serum
  5. Immunological Investigations (2020) IF: 2.511
    Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase-1/IL-1β Signaling Pathway Inhibition

    DOI: 10.1080/08820139.2020.1791178

    PMID: 32674625

    Sample: Cell culture supernatant
  6. Plos One (2015) IF: 2.806
    The Protective Role Of Interleukin-33 In Myocardial Ischemia And Reperfusion Is Associated With Decreased HMGB1 Expression And Up-Regulation Of The P38 MAPK Signaling Pathway.

    DOI: 10.1371/journal.pone.0143064

    PMID: 26571038

    Sample: Serum,Tissue homogenate
  7. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research (2020) IF: 1.918
    Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

    DOI: 10.12659/MSM.919054

    PMID: 31992687

    Sample: Plasma
  8. Int J Recent Sci Res (2015)
    Interleukin-17: a cardiac biomarker in estimation of cardioprotective effects of tacrolimus in doxorubicin-induced cardiotoxicity: animal model study

    PMID: 99999999

    Sample: Serum
  9. International Journal of Clinical and Experimental Pathology (2020) IF: 0.252
    Sepsis causes heart injury through endoplasmic reticulum stress-mediated apoptosis signaling pathway

    PMID: 32509067

    Sample: Serum
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