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Rat TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit

  • Cat.No.:E-EL-R1253

  • Reactivity: Rat

To Purchase E-EL-R1253

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Rat TNNI3/cTn-I and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNNI3/cTn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNNI3/cTn-I. You can calculate the concentration of Rat TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TNNI3/cTn-I were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TNNI3/cTn-I were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 102.40 248.30 900.40 97.00 237.90 894.70
Standard deviation 5.70 14.90 36.90 5.10 11.40 37.60
CV (%) 5.57 6.00 4.10 5.26 4.79 4.20

Recovery

The recovery of Rat TNNI3/cTn-I spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-110 101
EDTA plasma (n=8) 93-111 101
Cell culture media (n=8) 93-107 100

Linearity

Samples were spiked with high concentrations of Rat TNNI3/cTn-I and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsTNNC1, cTnI, CMD1FF, CMD2A, CMH7, RCM1
Research AreaCancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. BIOMATERIALS (2021) IF: 12.479
    Polyphenol-assisted facile assembly of bioactive nanoparticles for targeted therapy of heart diseases

    DOI: 10.1016/j.biomaterials.2021.120952

    PMID: 34147720

    Sample: serum
  2. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
    Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

    DOI: 10.1186/s12951-022-01639-8

    PMID: 36195952

    Sample: blood
  3. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Cardioprotective Effects of Aconite in Isoproterenol-Induced Myocardial Infarction in Rats

    DOI: 10.1155/2022/1090893

    Sample: serum
  4. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Investigation of the impact of rosuvastatin and telmisartan in doxorubicin-induced acute cardiotoxicity

    DOI: 10.1016/j.biopha.2022.113673

    Sample: serum
  5. LIFE SCIENCES (2022) IF: 6.78
    Astragaloside IV ameliorates sepsis-induced myocardial dysfunction by regulating NOX4/JNK/BAX pathway

    DOI: 10.1016/j.lfs.2022.121123

    PMID: 36302499

    Sample: myocardium,serum
  6. PHYTOMEDICINE (2021) IF: 5.34
    Cardioprotective activity of ethyl acetate extract of Cinnamomi Ramulus against myocardial ischemia/reperfusion injury in rats via inhibiting NLRP3 inflammasome activation and pyroptosis

    DOI: 10.1016/j.phymed.2021.153798

    PMID: 34673348

    Sample: serum
  7. Journal of the American Heart Association (2021) IF: 5.501
    Post‐Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest

    DOI: 10.1161/JAHA.120.019837

    Sample: Plasma
  8. REDOX REPORT (2023) IF: 5.696
    Dexmedetomidine postconditioning attenuates myocardial ischemia/reperfusion injury by activating the Nrf2/Sirt3/SOD2 signaling pathway in the rats

    DOI: 10.1080/13510002.2022.2158526

    Sample: Serum
  9. Frontiers in Cardiovascular Medicine (2022) IF: 5.846
    Adenosine A2a Receptor Regulates Autophagy Flux and Apoptosis to Alleviate Ischemia-Reperfusion Injury via the cAMP/PKA Signaling Pathway

    DOI: 10.3389/fcvm.2022.755619

    Sample: cardiomyocytes
  10. ACTA PHARMACOLOGICA SINICA (2019) IF: 4.01
    Exogenous NADPH ameliorates myocardial ischemia–reperfusion injury in rats through activating AMPK/mTOR pathway

    DOI: 10.1038/s41401-019-0301-1

    Sample: Serum
  11. INFLAMMATION (2021) IF: 4.092
    MiR-708-3p Alleviates Inflammation and Myocardial Injury After Myocardial Infarction by Suppressing ADAM17 Expression

    DOI: 10.1007/s10753-020-01404-9

    PMID: 33492555

    Sample: Cell culture supernatant
  12. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats

    DOI: 10.1007/s11356-021-15409-w

    PMID: 34322808

    Sample: serum
  13. SAUDI PHARMACEUTICAL JOURNAL (2021) IF: 4.33
    Cinnamomum zeylanicum Blume (Ceylon cinnamon) bark extract attenuates doxorubicin induced cardiotoxicity in Wistar rats

    DOI: 10.1016/j.jsps.2021.06.004

    PMID: 34408544

    Sample: serum
  14. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 4.432
    Piperazine ferulate protects against cardiac ischemia/reperfusion injury in rat via the suppression of NLRP3 inflammasome activation and pyroptosis

    DOI: 10.1016/j.ejphar.2022.174856

    PMID: 35219728

    Sample: Myocardial
  15. MOLECULES (2022) IF: 4.927
    7-Hydroxyflavone Alleviates Myocardial Ischemia/Reperfusion Injury in Rats by Regulating Inflammation

    DOI: 10.3390/molecules27175371

    PMID: 36080137

    Sample: serum
  16. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats.

    DOI: 10.26355/eurrev_202006_21528

    Sample: Serum
  17. JOURNAL OF CELLULAR BIOCHEMISTRY (2017) IF: 3.085
    Functional and Structural Assessment of the Effect of Human Umbilical Cord Blood Mesenchymal Stem Cells in Doxorubicin‐Induced Cardiotoxicity

    DOI: 10.1002/jcb.26168

    Sample: Serum
  18. PLoS One (2015) IF: 3.24
    The Protective Role of Interleukin-33 in Myocardial Ischemia and Reperfusion Is Associated with Decreased HMGB1 Expression and Up-Regulation of the P38 MAPK Signaling Pathway

    DOI: 10.1371/journal.pone.0143064

    Sample: Serum,Tissue homogenate
  19. Life-Basel (2022) IF: 3.251
    Protective Effects of L-2-Oxothiazolidine-4-Carboxylate during Isoproterenol-Induced Myocardial Infarction in Rats: In Vivo Study

    DOI: 10.3390/life12101466

    PMID: 36294901

    Sample: plasma
  20. EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263
    Carbon monoxide attenuates LPS-induced myocardial dysfunction in rats by regulating the mitochondrial dynamic equilibrium

    DOI: 10.1016/j.ejphar.2020.173726

    PMID: 33159931

    Sample: Serum
  21. Bioengineered (2022) IF: 3.269
    Clemastine protects against sepsis-induced myocardial injury in vivo and in vitro

    DOI: 10.1080/21655979.2022.2047256

    PMID: 35274595

    Sample: serum
  22. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Tongmai Yangxin pills anti-oxidative stress alleviates cisplatin-induced cardiotoxicity: Network pharmacology analysis and experimental evidence

    DOI: 10.1016/j.biopha.2018.09.095

    Sample: serum
  23. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575
    Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

    DOI: 10.1016/j.bbrc.2018.02.121

    Sample: serum
  24. IMMUNOLOGICAL INVESTIGATIONS (2020) IF: 3.657
    Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase‑1/IL‑1β Signaling Pathway Inhibition

    DOI: 10.1080/08820139.2020.1791178

    Sample: Cell culture supernatant
  25. INFLAMMOPHARMACOLOGY (2019) IF: 3.838
    Urotensin-#receptor antagonist SB-706375 protected isolated rat heart from ischaemia–reperfusion injury by attenuating myocardial necrosis via RhoA/ROCK/RIP3 signalling pathway

    DOI: 10.1007/s10787-019-00598-1

    Sample: Bronchoalveolar Lavage Fluid
  26. BIOSCIENCE REPORTS (2022) IF: 3.976
    Role of Keap1-Nrf2/ARE signal transduction pathway in protection of dexmedetomidine preconditioning against myocardial ischemia/reperfusion injury

    DOI: 10.1042/BSR20221306

    PMID: 35959640

    Sample: serum
  27. BMC Cardiovascular Disorders (2022) IF: 2.174
    Assessing the risk factors for myocardial infarction in diet-induced prediabetes: myocardial tissue changes

    DOI: 10.1186/s12872-022-02758-8

    PMID: 35918636

    Sample: heart tissue
  28. Experimental and Therapeutic Medicine (2019) IF: 2.447
    H2S attenuates sepsis‑induced cardiac dysfunction via a PI3K/Akt‑dependent mechanism

    DOI: 10.3892/etm.2019.7440

    Sample: serum
  29. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Dynamic observation of 5‑fluorouracil‑induced myocardial injury and mitochondrial autophagy in aging rats

    DOI: 10.3892/etm.2021.10886

    Sample: serum
  30. Chinese Journal of Integrative Medicine (2022) IF: 2.626
    Shenmai Injection Attenuates Myocardial Ischemia/Reperfusion Injury by Targeting Nrf2/GPX4 Signalling-Mediated Ferroptosis

    DOI: 10.1007/s11655-022-3620-x

    PMID: 35997859

    Sample: blood
  31. Pharmacological Reports (2019) IF: 2.761
    Bisoprolol and linagliptin ameliorated electrical and mechanical isometric myocardial contractions in doxorubicin-induced cardiomyopathy in rats

    DOI: 10.1007/s43440-019-00034-9

    Sample: plasma
  32. SCANDINAVIAN CARDIOVASCULAR JOURNAL (2020) IF: 1.084
    St. Thomas and del Nido cardioplegia are superior to Custodiol cardioplegia in a rat model of donor heart

    DOI: 10.1080/14017431.2020.1846772

    PMID: 33185130

    Sample: Tissue homogenate
  33. Emergency Medicine International (2015) IF: 1.112
    New Insights into the Role of Metformin Effects on Serum Omentin-1 Levels in Acute Myocardial Infarction: Cross-Sectional Study

    DOI: 10.1155/2015/283021

    Sample: serum
  34. MEDICAL SCIENCE MONITOR (2020) IF: 1.918
    Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

    DOI: 10.12659/MSM.919054

    PMID: 31992687

    Sample: Plasma
  35. International Journal of Clinical and Experimental Pathology (2020) IF: 0.252
    Sepsis causes heart injury through endoplasmic reticulum stress-mediated apoptosis signaling pathway

    PMID: 32509067

    Sample: serum
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