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Reactive Oxygen Species (ROS) Fluorometric Assay Kit

  • Cat.No.:E-BC-K138-F

  • Detection instrument: Fluorescence Microplate reader (Ex/Em=500 nm/525 nm), Fluorescence Spectrophotometer (Ex/Em=500 nm/5

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  • 96T
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Detection principle

DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

Performance characteristics

Synonyms ROS
Sample type Fresh tissue,cultured cells
Detection method Fluorescence method
Assay type Cell-based (quantitative)
Assay time 60 min
Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Storage Reagent 1: -20℃, Reagent 2: 2-8℃
Valid period 6 months

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cardiovascular Drugs and Therapy (2020) IF: 4.069
    Exendin-4 Attenuates Remodeling in the Remote Myocardium of Rats After an Acute Myocardial Infarction by Activating β-Arrestin-2, Protein Phosphatase 2A, and Glycogen Synthase Kinase-3 and Inhibiting β-Catenin

    DOI: 10.1007/s10557-020-07006-9

    PMID: 32474680

    Sample: Tissue homogenate
  2. Plant Archives (2019) IF: 0.1
    STUDY OF PURIFIED MICROCYSTIN-LR TOXICITY ON BIOCHEMICAL MARKERS IN BLOOD AND LIVER OF CYPRINUS CARPIO DURING ACUTE EXPOSURE

    PMID: 99999999

    Sample: Tissue homogenate
  3. Preprint (2019)
    Evaluating the Bystander Effects of Electron Beam Irradiation in Radiotherapy on MCF-7 Human Breast Cancer Cells

    DOI: 10.21203/rs.2.17986/v1

    PMID: 99999999

    Sample: Cell sample
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Reviews/Q&A

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Q

a********dSubmitted [ Apr 06 2021 ]

Asked: What is the procedure for this assay?

Reply

A

adminSubmitted [ Apr 06 2021 ]

Answered: Hi, Thanks for your interests in our products. There are detailed operation in the manual. Can we know where didn't we make clear? If you need any help, pls feel free to ask. Best regards, Hanny

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