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Cat.No.:PKSR030500
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Synonyms | 2019-nCov S protein, 2019-nCoV Spike glycoprotein, 2019-nCoV S glycoprotein |
Species | SARS-CoV-2 |
Expression_host | HEK293 Cells |
Sequence | Arg319-Phe541 |
Accession | YP_009724390.1 |
Application | Immunogen(E-AB-V1006,E-AB-V1025) |
Mol_Mass | 51.5 kDa |
Tag | C-mFc |
Bio_Activity | 1. Immobilized human ACE2 protein (His tag) (Cat#PKSR030508) at 2μg/mL (100μL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) PKSR030500, the EC50 of Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50 ng/mL. 2. Loaded Human ACE2 Protein (His tag) (Cat#PKSR030508) on SA Biosensor, can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) (Cat. No. PKSR030500) with an affinity constant of 0.562 nM as determined by Octet RED System. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | LyopHilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
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