Recombinant ALDH3A1 Monoclonal Antibody (AN300160P)
For research use only.
| Verified Samples | Verified Samples in WB:A549 |
| Dilution | WB: 1:1000-1:5000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human ALDH3A1 Protein |
| Abbre | ALDH3A1 |
| Synonyms | ALDH, ALDH3A, ALDH3, ALDH3A1, Acetaldehyde dehydrogenase 3, AHD 4, AHD C, AHD4, AHDC, AL3A1, Aldehyde dehydrogenase, Aldehyde dehydrogenase 3, Aldehyde dehydrogenase 3 family member A1, Aldehyde dehydrogenase 3A1, Aldehyde dehydrogenase class 3, Aldehyde dehydrogenase dimeric NADP preferring, Aldehyde dehydrogenase family 3, Aldehyde dehydrogenase family 3 member A1, Aldehyde dehydrogenase family 3 subfamily A1, Aldehyde dehydrogenase isozyme 3, Aldehyde dehydrogenase type III, ALDH 3, ALDH 3A1, ALDH III, ALDH3 A1, ALDH3A 1, ALDH3A10, ALDHIII, dimeric NADP-preferring, member 1, MGC10406, Stomach aldehyde dehydrogenase, stomach type, subfamily A, Tumor associated aldehyde dehydrogenase tumor ALDH |
| Swissprot | |
| Calculated MW | 50 kDa |
| Observed MW |
50 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Neuroscience, Cell Biology, Metabolism |
| Clone | A1274 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008] |
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