Recombinant Angiotensinogen/AGT Monoclonal Antibody (AN300511P)

Name: Recombinant Angiotensinogen/AGT Monoclonal Antibody
Brand: Elabscience
SKU: AN300511P
Price: 380.0 USD
Availability: InStock

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Recombinant Angiotensinogen/AGT Monoclonal Antibody - 1

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	100μL	$ 380.00	- +

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Verified Samples	Verified Samples in WB: PC-12, HepG2, U2OS, C2C12
Dilution	WB 1:1000, IP 1:20-1:50
Isotype	IgG
Host	Rabbit
Reactivity	Human, Mouse, Rat
Applications	WB, IP
Clonality	Monoclonal
Immunogen	Recombinant Mouse Angiotensinogen/AGT Protein
Abbre	AGT
Synonyms	Serpina, AI265500, AngI, AngII, ANHU, Aogen, Serpina8, Agt, AGT, AI265500, Alpha 1 antiproteinase antitrypsin, Ang, Ang I, Ang II, Ang III, AngII, Angiotensin I, Angiotensin II, Angiotensin III, Angiotensin-3, Angiotensinogen, Angiotensinogen (PAT), ANGT, ANHU, ANRT, AT-2, AT-II, Des-Asp[1]-angiotensin II, PAT, Pre angiotensinogen, Serine (or cysteine) proteinase inhibitor, Serpin A8, Serpin peptidase inhibitor clade A member 8, SERPINA8, Aangiotensinogen (serpin peptidase inhibitor clade A member 8), Angiotensin 1-7, Angiotensin 1-9, Angiotensin-1
Swissprot	P01019
Calculated MW	51 kDa
Observed MW	51 kDa,60 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Secreted
Tissue Specificity	Expressed by the liver and secreted in plasma.
Concentration	1 mg/mL
Buffer	0.2 μm filtered solution in PBS
Purification Method	Affinity Purification
Clone	B441
Conjugation	Unconjugated
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Shipping	Ice bag
background	The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008]