Recombinant CD55/DAF Monoclonal Antibody (AN300017P)
For research use only.
| Verified Samples | Verified Samples in WB:Hela |
| Dilution | WB: 1:2000-1:20000；IHC-P: 1:200-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human CD55 protein |
| Abbre | CD55 |
| Synonyms | CD55, CR, CROM, DAF, TC, Complement Decay-Accelerating factor, CD 55, CD55 antigen, CD55 Cromer blood group system, CD55 molecule, CD55 molecule (Cromer blood group), Cd55a, Complement decay accelerating factor, Cromer Blood Group antigen, Cromer blood group system, Cromer blood group system), Daf1, Daf-GPI, Dcay accelerating factor for complement (CD55, Decay accelarating factor 1, Decay accelerating factor (GPI-form), Decay Accelerating Factor for Complement, decay accelerating factor for complement (Cromer blood group), Decay accelerating factor GPI-form, Decay accelerating factor soluble-form, GPI-anchored, GPI-DAF, isoform CRA_a |
| Swissprot | |
| Calculated MW | 41 kDa |
| Observed MW |
78 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology |
| Clone | A1127 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014] |
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