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Recombinant Dechloromonas aromatica Chlorite Dismutase Protein (His Tag)

  • Cat.No.:PKSQ050054

  • Expression host: E.coli

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Description

Synonyms Chlorite dismutase; Chlorite O(2)-lyase; Daro_2580; Cld
Species Dechloromonas aromatica
Expression_host E.coli
Sequence Met35-Asp282
Accession Q47CX0
Mol_Mass 31.3 kDa
AP_Mol_Mass 32 kDa
Tag N-His

Properties

Purity > 95% as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg as determined by the LAL method.
Storage Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, 0.5mM EDTA, pH7.4.
Reconstitution Please refer to the printed manual for detailed information.

Background

Chlorite dismutase (Cld) found in prokaryotic organisms, also known as Chlorite O2-lyase, is a b-type heme containing enzyme that catalyzes the reduction of chlorite into chloride plus dioxygen. The subunit of chlorite dismutase consists of a heme free N-terminal and a heme b containing C-terminal ferredoxin-like fold with high structural homology to the dye-decolorizing peroxidases (DyPs). The physiological role of Cld in prokaryote has been shown that some microorganisms can use perchlorate or chlorate as terminal electron acceptors for anaerobic respiration thereby producing chlorite that must be detoxified. This enzyme has gained attention because it can be used in the development of bioremediation processes, biosensors, and controlled dioxygen production.

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