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Recombinant Human 4-1BBL/TNFSF9 Protein (His Tag)(Active)

  • Cat.No.:PKSH032023

  • Expression host: E.coli

To Purchase PKSH032023

Size:
  • 10μg
  • 50μg
Price: $129
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Description

Synonyms Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; TNFSF9
Species Human
Expression_host E.coli
Sequence Arg71-Glu254
Accession P41273
Application Functional ELISA
Mol_Mass 41.8 kDa
AP_Mol_Mass 16-20 kDa
Tag C-6His
Bio_Activity Immobilized Human 4-1BB-His(Cat: PKSH032025) at 10μg/ml(100 μl/well) can bind Biotinylated Human 4-1BBL-His. The ED50 of Human 4-1BBL-His is 147.3 ug/ml .

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Reconstitution Please refer to the printed manual for detailed information.

Background

Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain, placenta, lung, skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction.

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