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Recombinant Human Autotaxin/ENPP2 Protein (aa 36-863, His Tag)

Uniprot : Q13822

Cat.No.:PKSH033693
Expression host: HEK293 Cells

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Description

Synonyms	ATX;ATXFLJ26803;ATX-X;Autotaxin;autotaxin-t;EC 3.1.4.39;ectonucleotide pyrophosphatase/phosphodiesterase 2;E-NPP 2;ENPP2;LysoPLD;NPP2;PD-IALPHA;PDNP2;PDNP2NPP2
Species	Human
Expression_host	HEK293 Cells
Sequence	Ala36-Ile863
Accession	AAH34961.1
Mol_Mass	96 kDa
AP_Mol_Mass	100-120 kDa
Tag	C-His

Properties

Purity	> 90 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mMPB,150mMNaCl,pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain.Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.

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