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Recombinant Human CD27/TNFRSF7 Protein (His Tag)(Active)

  • Cat.No.:PKSH031925

  • Expression host: Baculovirus-Insect Cells

To Purchase PKSH031925

Size:
  • 100μg
  • 200μg
Price: $454
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Description

Synonyms S152;S152. LPFS2;S152.LPFS2;T14;TNFRSF7;Tp55
Species Human
Expression_host Baculovirus-Insect Cells
Sequence Met 1-Ile192
Accession P26842
Application Functional ELISA
Mol_Mass 20.7 kDa
AP_Mol_Mass 27 kDa
Tag C-His
Bio_Activity Measured by its binding ability in a functional ELISA.Immobilized human CD27-his at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 57-85 ng/mL.

Properties

Purity > 85 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Reconstitution Please refer to the printed manual for detailed information.

Background

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

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