Synonyms	Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA;PECA1;CD31;PECAM1
Species	Human
Expression_host	HEK293 Cells
Sequence	Met 1-Lys 601
Accession	EAW94208.1
Mol_Mass	91.2 kDa
AP_Mol_Mass	130-140 kDa
Tag	C-hFc
Bio_Activity	Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 10⁴ cells/well are added to sCD31-Fc coated plates (1. 25μg/mL, 100μL/well) in the presence of 20ng/mL PMA, approximately 20-45% will adhere after 30 minutes at 37℃.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

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