Recombinant Human CDK2AP2 Protein (E.coli, His Tag) (PKSH030818)
For research use only.
| Synonyms | CDK2-associated protein 2, CDK2AP2, Cyclin-dependent kinase 2-associated protein 2, DOC-1-related protein, DOC-1R, DOC1R, p14 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Thr 126 |
| Accession | O75956 |
| Calculated Molecular Weight | 14.5 kDa |
| Observed Molecular Weight | 18&12 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS, 15% glycerol, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | CDK2AP2 belongs to the CDK2AP family. Members of this family of proteins are cell-growth suppressors; associating with and influencing the biological activities of important cell cycle regulators in the S phase including monomeric non-phosphorylated cyclin-dependent kinase 2 (CDK2) and DNA polymerase alpha/primase. CDK2AP2 contains 5 distinct gt-ag introns. Transcription produces 7 different mRNAs; 6 alternatively spliced variants and 1 unspliced form. There are 2 non overlapping alternative last exons and 4 validated alternative polyadenylation sites. The mRNAs appear to differ splicing versus retention of 3 introns. CDK2AP2 plays a role in regulating self-renewal of mouse embryonic stem cells (mESC) under permissive conditions; and cell survival during differentiation of the mESC into terminally differentiated cell types. |
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