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Recombinant Human Coagulation Factor X/F10 Protein (Fc Tag)

Uniprot : P00742

Cat.No.:PKSH033714
Expression host: HEK293 Cells

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Description

Synonyms	Coagulation factor 10;coagulation factor X;FX;FXA
Species	Human
Expression_host	HEK293 Cells
Sequence	Asn32-Lys488
Accession	P00742
Mol_Mass	78.2 kDa
AP_Mol_Mass	80-120&25 kDa
Tag	C-Fc

Properties

Purity	> 90 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 0.2mM CaCl₂, pH 5.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer infor
Reconstitution	Please refer to the printed manual for detailed information.

Background

F10, also known as Coagulation factor X, belongs to the peptidase S1 family that is synthesized as a 488 amino acid (aa) with a signal peptide and a pro region (residues 1-40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41-179) and heavy (residues 235-488) chains linked by a disulfide bond. Coagulation factor X is initially synthesized in the liver. The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds. Forms a heterodimer with SERPINA5. F10 is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

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