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Recombinant Human DDR2 Kinase/CD167b Protein (aa 422-855, His & GST Tag)

Cat:PKSH030420
Manual MSDS

Price: $ 717

Size:
50μg
Quantity:
  • Expression Host: Baculovirus-Insect Cells
  • Accession: Q16832
  • Applications: Enzyme
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Product Details
Synonyms CD167;MIG20a;NTRKR3;TKT;TYRO10
Species Human
Expression Host Baculovirus-Insect Cells
Sequence Arg422-Glu855
Accession Q16832
Calculated Molecular Weight 77.1 kDa
Observed Molecular Weight 77 kDa
Tag N-His-GST
Bio-activity The specific activity was determined to be 8 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
Reconstitution Not Applicable
Background Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.