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Cat.No.:PKSH030706
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Synonyms | FCE1A;FcERI |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met 1-Gln205 |
Accession | P12319 |
Mol_Mass | 22.5 kDa |
AP_Mol_Mass | 42-48 kDa |
Tag | C-His |
Bio_Activity | Immobilized Recombinant Human FcERI / FCER1A Protein (ECD,His Tag) at 2 μg/mL (100 μl/well) can bind Recombinant Human IgE-Fc Protein (Constant Domain 3&4, His Tag), the EC50 is 10-30 ng/mL. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
N**aSubmitted [ Apr 09 2024 ]
Asked: Hi, is it a single his tag or a multi his tag (e.g. 6x) Thank you!
Reply
adminSubmitted [ Apr 09 2024 ]
Answered: This protein uses a His tag with a composition of 6xHis.