Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155)(Q65I,C96S,N111G)

    • Recombinant Human FGF-2 / FGF basic / FGFb Protein(Met1-Ser155)(Q65I,C96S,N111G)-Elabscience
    • Recombinant Protein-Elabscience
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    • Recombinant Human FGF-2 / FGF basic / FGFb Protein(Met1-Ser155)(Q65I,C96S,N111G)-Elabscience
    • Recombinant Protein-Elabscience
    • Recombinant Human FGF-2 / FGF basic / FGFb Protein(Met1-Ser155)(Q65I,C96S,N111G)-Elabscience
    • Recombinant Protein-Elabscience

      Catalog number:PKSH033502

      Synonyms:Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB

      Size:
      • 10μg
      • 50μg
      Qty:
      - +
      Price: $86

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      Overview

      Synonyms Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
      Species Human
      Expression_host E.coli
      Sequence Met1-Ser155
      Accession P09038-2
      Mol_Mass 17.2 kDa
      AP_Mol_Mass 16 kDa

      Properties

      Purity > 95% as determined by reducing SDS-PAGE.
      Endotoxin < 1.0 EU per μg as determined by the LAL method.
      Storage Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
      Shipping This product is provided as lyophilized powder which is shipped with ice packs.
      Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris,200mM NaCl, pH7.5.
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule.

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