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Recombinant Human GFRA1/GDNFRA Protein (aa 25-429, His Tag)(Active)

Uniprot : P56159-2
  • Cat.No.:PKSH033670

  • Expression host: Human Cells

To Purchase PKSH033670

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  • 10µg
  • 50µg
Price: $49
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Description

Synonyms GDNF Family Receptor Alpha-1; GDNF Receptor Alpha-1; GDNFR-Alpha-1; GFR-Alpha-1; RET Ligand 1; TGF-Beta-Related Neurotrophic Factor Receptor 1; GFRA1; GDNFRA; RETL1; TRNR1
Species Human
Expression_host Human Cells
Sequence Asp25-Lys429
Accession P56159-2
Application Functional ELISA
Mol_Mass 46.28 kDa
AP_Mol_Mass 60 kDa
Tag C-His
Bio_Activity Immobilized Human GDNF(Cat: PKSH032488) at 1.5μg/ml(100 μl/well) can bind Human GFRA1-His. The ED50 of Human GFRA1-His is 0.468 ug/ml .

Properties

Purity > 90% as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg as determined by the LAL method.
Storage Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution Please refer to the printed manual for detailed information.

Background

Glial Cell Line-Derived Neurotrophic Factor Family Receptor α-1 (GDNFRα1) is a glycosylphosphatidylinositol (GPI) linked cell surface protein belonging to GDNF-family receptor α subtype which consists of at least four members. GFRα1and GFRα2 are the cognate co-receptor for the neurotrophic factor neurturin mediating the NRTN-induced autophosphorylation and activation of the RET tyrosine kinase receptor. Soluble GFRαs released enzymatically from the cell surface by phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFRα1, can also bind with high affinity to GDNF and trigger the activation of Ret tyrosine kinase. Human GFRα1 shares 93% amino acid identity with mouse GFRα1.The expression of the various GFRαs are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFRαs in mediating the activities of the GDNF family of neurotrophic factors.

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