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Cat.No.:PKSH030425
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Synonyms | GSK3B |
Species | Human |
Expression_host | Baculovirus-Insect Cells |
Sequence | Met 1-Thr 433 |
Accession | NP_002084.2 |
Application | Functional ELISA |
Mol_Mass | 50.4 kDa |
AP_Mol_Mass | 44-48 kDa |
Tag | N-His |
Bio_Activity | 1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTA |
Reconstitution | Not Applicable |