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Recombinant Human/Mouse FGF-8b/FGF8B Protein

Uniprot : P55075&P37237
  • Cat.No.:PKSH033805

  • Expression host: E.coli

To Purchase PKSH033805

Size:
  • 10μg
  • 50μg
Price: $129
Qty:

Description

Synonyms Fibroblast growth factor 8;Androgen-induced growth factor;Heparin-binding growth factor 8;AIGF;HBGF-8;FGF-8B
Species Human/Mouse
Expression_host E.coli
Sequence Gln23-Arg215
Accession P55075-3/P37237-2
Application Cell Culture
Mol_Mass 22.5 kDa
AP_Mol_Mass 23 kDa
Tag None
Bio_Activity Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 21.87 ng/ml.

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 0.01 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB,300mM NaCl,2% Sucrose,0.02% Tween 80,pH7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

Fibroblast growth factor 8 (FGF-8) is a member of the fibroblast growth factor family. It is discovered as a growth factor essential for the androgen--dependent growth of mouse mammary carcinoma cells. Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial--mesenchymal transitions. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. It is required for normal brain, eye, ear, limb development during embryogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

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