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Recombinant Human/Mouse FGF-8b/FGF8B Protein (Active)

Uniprot : P55075-3/P37237-2

Cat.No.:PKSH033805
Expression host: E.coli

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Description

Synonyms	Fibroblast growth factor 8, Androgen-induced growth factor, Heparin-binding growth factor 8, AIGF, HBGF-8, FGF-8B
Species	Human/Mouse
Expression_host	E.coli
Sequence	Gln23-Arg215
Accession	P55075-3/P37237-2
Application	Cell Culture
Mol_Mass	22.5 kDa
AP_Mol_Mass	21 kDa
Tag	None
Bio_Activity	Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 10-60 ng/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Fibroblast growth factor 8 (FGF-8) is a member of the fibroblast growth factor family. It is discovered as a growth factor essential for the androgen--dependent growth of mouse mammary carcinoma cells. Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial--mesenchymal transitions. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. It is required for normal brain, eye, ear, limb development during embryogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

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