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Recombinant Human/Mouse/Rat Irisin/FNDC5 Protein (His Tag)

Cat:PKSH033461 Citations (1)
Manual MSDS

Price: $ 375

Price: $ 129

Size:
50μg 10μg
Quantity:
  • Expression Host: HEK293 Cells
  • Accession: Q8NAU1
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Product Details
Synonyms Fibronectin type III domain-containing protein 5;Fibronectin type III repeat-containing protein 2;Irisin;FNDC5
Species Human/Mouse/Rat
Expression Host HEK293 Cells
Sequence Asp32-Glu143
Accession Q8NAU1
Calculated Molecular Weight 13.4 kDa
Observed Molecular Weight 20-28 kDa
Tag C-His
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Fibronectin type III domain-containing protein 5; the precursor of irisin; is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD); a 21 aa transmembrane domain; and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human; mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1(UCP1); mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.