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Recombinant Human Osteoprotegerin/TNFRSF11B Protein (Fc Tag)(Active)

  • Cat.No.:PKSH033124

  • Expression host: Human Cells

To Purchase PKSH033124

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  • 10μg
  • 50μg
Price: $178
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Description

Synonyms Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; OCIF; OPG;PDB5;TR1
Species Human
Expression_host Human Cells
Sequence Glu22-Leu201
Accession O00300
Application Functional ELISA
Mol_Mass 47.2 kDa
AP_Mol_Mass 50-80 kDa
Tag C-Fc
Bio_Activity Immobilized Human OPG-Fc at 2μg/ml(100 μl/well) can bind Mouse RANKL-His(Cat: PKSM041165). The ED50 of Mouse OPG-Fc is 2.44ug/ml .

Properties

Purity > 90 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Reconstitution Please refer to the printed manual for detailed information.

Background

TNFRSF11B is a secreted protein, containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). By binding RANKL, TNFRSF11B inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.

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