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Cat.No.:PKSH031027
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Synonyms | CD254;ODF;OPGL;RANKL;TNFSF11;CD254;Osteoclast differentiation factor;Receptor activator of nuclear factor kappa-B ligand;tumor necrosis factor ligand superfamily member 11;hRANKL2;OPTB2;RANKL;sOdf |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Gly 64-Asp 245 |
Accession | AAC51762.1 |
Application | Functional ELISA |
Mol_Mass | 20.5 kDa |
AP_Mol_Mass | 27 kDa |
Tag | None |
Bio_Activity | 1. Immobilized human TNFSF11 at 2 μg/ml (100 μl/well) can bind biotinylated human Osteoprotegerin hFc, The EC50 of human Osteoprotegerin hFc is 5-40 ng/ml. 2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264. 7 cells. The ED50 for this effect is typically 1.5-7.5 ng/mL. |
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |