Recombinant Ly108/SLAMF6 Monoclonal Antibody (AN300021P)
For research use only.
| Verified Samples |
Verified Samples in WB: Ramos in IF: Jurkat |
| Dilution | WB: 1:1000；ICC/IF: 1:500-1:1000；FC: 1:200-1:500；IP: 1:20-1:50 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, ICC/IF, FC, IP |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human Ly108 / SLAMF6 protein |
| Abbre | SLAMF6 |
| Synonyms | UNQ, SLAM Family Member, SLAMF, SLAMF6, CD352, KALI, KALIb, Ly108, NTB-A, NTBA, SF2000, Activating NK Receptor, NK-T-B-Antigen, SLAM Family Member 6, UNQ6123, PRO20080, NTB-A, KAL1, Ly108 |
| Swissprot | |
| Calculated MW | 37 kDa |
| Observed MW |
60 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Tissue Specificity | Expressed by all (resting and activated) natural killer cells (NK), T- and B-lymphocytes. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology |
| Clone | A1131 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a type I transmembrane protein, belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK), T, and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010] |
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