Recombinant Mouse Cathepsin B/CTSB Protein (His Tag)

    • Recombinant Mouse  Cathepsin B Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
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    • Recombinant Mouse  Cathepsin B Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
    • Recombinant Mouse  Cathepsin B Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience

      Catalog number:PKSM040933

      Synonyms:Cathepsin B;Ctsb;Cathepsin B1

      Size:
      • 10 μg
      • 20 μg
      Qty:
      - +
      Price: $454

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Overview

      Synonyms Cathepsin B;Ctsb;Cathepsin B1
      Species Mouse
      Expression_host HEK293 Cells
      Sequence Met 1-Phe 339
      Accession P10605
      Mol_Mass 36.6 kDa
      Tag C-His
      Bio_Activity Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The specific activity is >2,000 pmoles/min/µg.

      Properties

      Purity > 95 % as determined by SDS-PAGE
      Endotoxin < 1.0 EU per µg of the protein as determined by the LAL method.
      Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
      Shipping This product is provided as lyophilized powder which is shipped with ice packs.
      Formulation Lyophilized from sterile PBS, pH 7.4
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

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