Recombinant Mouse CD40LG/TNFSF5 Protein (His Tag)(Active)

    • Recombinant Mouse CD40L / CD154 / TNFSF5 Protein (N-His)-Elabscience
    • Recombinant Protein-Elabscience
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    • Recombinant Mouse CD40L / CD154 / TNFSF5 Protein (N-His)-Elabscience
    • Recombinant Protein-Elabscience
    • Recombinant Mouse CD40L / CD154 / TNFSF5 Protein (N-His)-Elabscience
    • Recombinant Protein-Elabscience

      Catalog number:PKSM041352

      Synonyms:CD40 Ligand; CD40LG;HIGM1; T-B cell-activating molecule; T-BAM; TNFSF5; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

      Size:
      • 10μg
      • 50μg
      Qty:
      - +
      Price: $111

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Overview

      Synonyms CD40 Ligand; CD40LG;HIGM1; T-B cell-activating molecule; T-BAM; TNFSF5; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5
      Species Mouse
      Expression_host Human Cells
      Sequence Met112-Leu260
      Accession P27548
      Application Functional ELISA
      Mol_Mass 18.8 kDa
      AP_Mol_Mass 20 kDa
      Tag N-His
      Bio_Activity Immobilized Human CD40-Fc(Cat: PKSM041225) at 2μg/ml(100 μl/well) can bind Human CD40LG-His. The ED50 of Human CD40LG-His(Cat: PKSH033600)) is 13.84ng/mL.

      Properties

      Purity > 95% as determined by reducing SDS-PAGE.
      Endotoxin < 1.0 EU per μg as determined by the LAL method.
      Storage Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
      Shipping This product is provided as lyophilized powder which is shipped with ice packs.
      Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.

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      MSDS for ELISA ISO 9001

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