English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

Recombinant Mouse Cystatin 7/CST7 Protein (Fc Tag)

Cat:PKSM040785
Manual MSDS

Price: $ 612

Size:
20μg
Quantity:
  • Expression Host: HEK293 Cells
  • Accession: O89098
  • Applications: Enzyme
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Synonyms Cystatin-F;Cystatin-like Metastasis-Associated Protein;Leukocystatin;CMAP;Cystatin-7;Cst7
Species Mouse
Expression Host HEK293 Cells
Sequence Met1-Gln144
Accession O89098
Calculated Molecular Weight 41.4 kDa
Observed Molecular Weight 42 kDa
Tag C-hFc
Bio-activity Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is < 6 nM.
Purity > 93 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 25mM Tris, 0.15M NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.