Recombinant Mouse Cystatin 7/CST7 Protein (Fc Tag)
Price: $ 612
Size:
20μg
Quantity:
- Expression Host: HEK293 Cells
- Accession: O89098
- Applications: Enzyme
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Product Details
Synonyms | Cystatin-F;Cystatin-like Metastasis-Associated Protein;Leukocystatin;CMAP;Cystatin-7;Cst7 |
Species | Mouse |
Expression Host | HEK293 Cells |
Sequence | Met1-Gln144 |
Accession | O89098 |
Calculated Molecular Weight | 41.4 kDa |
Observed Molecular Weight | 42 kDa |
Tag | C-hFc |
Bio-activity | Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is < 6 nM. |
Purity | > 93 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
Lyophilized from sterile 25mM Tris, 0.15M NaCl, pH 7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis. |