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Recombinant Mouse ICOS Ligand/ICOSL Protein (His Tag)

Cat:PKSM040815
Manual MSDS

Price: $ 670

Price: $ 5237

Size:
100μg 1mg
Quantity:
  • Expression Host: HEK293 Cells
  • Accession: NP_056605.1
  • Applications: ELISA
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Product Details
Synonyms Icosl;AU044799;B7-H2;B7RP-1;B7h;BG071784;GI50;GL50;GL50-B;ICOS-L;KIAA0653;LICOS;Ly115l;Mkiaa0653
Species Mouse
Expression Host HEK293 Cells
Sequence Met 1-Lys 279
Accession NP_056605.1
Calculated Molecular Weight 27.8 kDa
Observed Molecular Weight 45-55 kDa
Tag C-His
Bio-activity Immobilized mouse B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 40-1000 ng/ml.
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.