Recombinant Mouse Interleukin-33/IL-33 Protein
Price: $ 739
Price: $ 249
Size:
100μg
20μg
Quantity:
- Expression Host: E.coli
- Accession: Q8BVZ5
- Applications: Cell culture
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Product Details
Synonyms | Interleukin 33;IL-33;IL33;C9orf26;NKHEV;Interleukin-1 family member 11;DVS27;NF-HEV and IL- 1F11 |
Species | Mouse |
Expression Host | E.coli |
Sequence | Ser109-Ile266 |
Accession | Q8BVZ5 |
Calculated Molecular Weight | 18.5 kDa |
Observed Molecular Weight | 17-25 kDa |
Tag | C-His |
Bio-activity | Measure by its ability to induce proliferation in D10.G4.1 cells. The ED<sub>50</sub> for this effect is <40 pg/mL. The specific activity of recombinant mouse IL-33 is > 2 x 10<sup>7</sup> IU/mg. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells. IL-33 is constitutively expressed in smooth muscle and airway epithelia. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs. |