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Recombinant Mouse Interleukin-33/IL-33 Protein

Cat:PKSM041090
Manual MSDS

Price: $ 739

Price: $ 249

Size:
100μg 20μg
Quantity:
  • Expression Host: E.coli
  • Accession: Q8BVZ5
  • Applications: Cell culture
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Product Details
Synonyms Interleukin 33;IL-33;IL33;C9orf26;NKHEV;Interleukin-1 family member 11;DVS27;NF-HEV and IL- 1F11
Species Mouse
Expression Host E.coli
Sequence Ser109-Ile266
Accession Q8BVZ5
Calculated Molecular Weight 18.5 kDa
Observed Molecular Weight 17-25 kDa
Tag C-His
Bio-activity Measure by its ability to induce proliferation in D10.G4.1 cells. The ED<sub>50</sub> for this effect is <40 pg/mL. The specific activity of recombinant mouse IL-33 is > 2 x 10<sup>7</sup> IU/mg.
Purity > 98 % as determined by reducing SDS-PAGE.
Endotoxin < 0.1 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells. IL-33 is constitutively expressed in smooth muscle and airway epithelia. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs.