Recombinant Mouse SerpinD1/HCF2 Protein (His Tag)(Active)

    • Recombinant Mouse SerpinD1 / HCII Protein (His tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
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    • Recombinant Mouse SerpinD1 / HCII Protein (His tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
    • Recombinant Mouse SerpinD1 / HCII Protein (His tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience

      Catalog number:PKSM040854

      Synonyms:Heparin cofactor 2; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; Serpin D1

      Size:
      • 10 μg
      • 20 μg
      Qty:
      - +
      Price: $454

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Overview

      Synonyms Heparin cofactor 2; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; Serpin D1
      Species Mouse
      Expression_host HEK293 Cells
      Sequence Met1-Ser 478
      Accession NP_032249.3
      Mol_Mass 53.5 kDa
      AP_Mol_Mass 65-70 kDa
      Tag C-His
      Bio_Activity Measured by its ability to inhibit thombin (Sigma, T4648) cleavage of fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catslog# ES011). The IC50 value is < 1.2 nM.

      Properties

      Purity > 98 % as determined by SDS-PAGE
      Endotoxin < 1.0 EU per µg of the protein as determined by the LAL method.
      Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
      Shipping This product is provided as lyophilized powder which is shipped with ice packs.
      Formulation Lyophilized from sterile 25mM HEPES, 150mM NaCl, pH 7.4
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

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