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Reduced Glutathione (GSH) Colorimetric Assay Kit

  • Cat.No.:E-BC-K030-M

  • Detection instrument: Microplate reader(405-414 nm)

To Purchase E-BC-K030-M

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  • 96T
  • 48T
Price: $150
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Protocols for metabolism assays

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Detection principle

Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.

Performance characteristics

Synonyms GSH
Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 2 μmol/L
Detection range 2-100 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 25 min
Precision Average inter-assay CV: 3.200%Average intra-assay CV: 1.900%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

Significant differences can be observed comparing with P group. (*P<0.05, **P

G G Liou et al found that N-acetyl cysteine can reduce propacetamol-induced hepatotoxicity with low dose, but induce with high dose. Reduced glutathione level of mouse liver tissue was determined using GSH colorimetric assay kit (E-BC-K030-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

10% Mouse brain tissue homogenization

1

10% Mouse liver tissue homogenization

1

Hela cell  homogenization0.999 mgprot/mL

1

Rat serum

1

Rat plasma

1

Mouse serum

1

10% Carrot tissue homogenization

1

293T supernatant

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. CHEMICAL ENGINEERING JOURNAL (2022) IF: 16.744
    Tumor microenvironment activated nanoreactors for chemiluminescence imaging-guided simultaneous elimination of breast tumors and tumor-resident intracellular pathogens

    DOI: 10.1016/j.cej.2022.139939

    Sample: MCF-7 cells
  2. SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
    Size-dependent acute toxicity and oxidative damage caused by cobalt-based framework (ZIF-67) to Photobacterium phosphoreum

    DOI: 10.1016/j.scitotenv.2022.158317

    PMID: 36037900

    Sample: T3 strain
  3. Acta Biomaterialia (2022) IF: 8.9469
    An all-in-one biomimetic iron-small interfering RNA nanoplatform induces ferroptosis for cancer therapy

    DOI: 10.1016/j.actbio.2022.06.017

    PMID: 35709941

    Sample: CAL-27 cell
  4. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling Pathway

    DOI: 10.1155/2022/3593294

    PMID: 36238648

    Sample: hippocampus
  5. Antioxidants (2022) IF: 7.675
    Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

    DOI: 10.3390/antiox11112105

    PMID: 36358477

    Sample: liver,serum
  6. Antioxidants (2022) IF: 7.675
    Resistance Training Modulates Reticulum Endoplasmic Stress, Independent of Oxidative and Inflammatory Responses, in Elderly People

    DOI: 10.3390/antiox11112242

    Sample: PBMCs
  7. Antioxidants (2022) IF: 7.675
    Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

    DOI: 10.3390/antiox11101879

    PMID: 36290602

    Sample: ovarian tissue
  8. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids

    DOI: 10.1016/j.scitotenv.2021.150328

    PMID: 34571217

    Sample: liver organoids (LOs)
  9. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
    Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

    DOI: 10.1016/j.fct.2022.113061

    PMID: 35489465

    Sample: pig testicular cell (ST cell)
  10. Biomedicines (2021) IF: 6.081
    WIN55,212-2 Attenuates Cognitive Impairments in AlCl3 + d-Galactose-Induced Alzheimer’s Disease Rats by Enhancing Neurogenesis and Reversing Oxidative Stress

    DOI: 10.3390/biomedicines9091270

    Sample: brain
  11. Cell Death & Disease (2020) IF: 6.304
    Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload

    DOI: 10.1038/s41419-020-02777-3

    Sample: heart
  12. Antioxidants (2022) IF: 6.313
    Potential Neurotoxic Effects of Glioblastoma-Derived Exosomes in Primary Cultures of Cerebellar Neurons via Oxidant Stress and Glutathione Depletion

    DOI: 10.3390/antiox11071225

    PMID: 35883716

    Sample: T98G cell,U373 cell
  13. Antioxidants (2021) IF: 6.313
    N-Acetyl Cysteine Overdose Inducing Hepatic Steatosis and Systemic Inflammation in Both Propacetamol-Induced Hepatotoxic and Normal Mice

    DOI: 10.3390/antiox10030442

    PMID: 33809388

    Sample: Tissue homogenate
  14. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
    Carboxymethyl cellulose/sericin-based hydrogels with intrinsic antibacterial, antioxidant, and anti-inflammatory properties promote re-epithelization of diabetic wounds in rats

    DOI: 10.1016/j.ijpharm.2022.122328

    PMID: 36280221

    Sample: skin tissues
  15. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation

    DOI: 10.1016/j.biopha.2021.112467

    PMID: 34891114

    Sample: serum
  16. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats

    DOI: 10.1016/j.biopha.2021.112017

    PMID: 34399203

    Sample: heart
  17. MOLECULAR NUTRITION & FOOD RESEARCH (2022) IF: 6.575
    Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis

    DOI: 10.1002/mnfr.202200341

    PMID: 36069237

    Sample: colon tissue
  18. Cells (2021) IF: 6.6
    Loganin Ameliorates Painful Diabetic Neuropathy by Modulating Oxidative Stress, Inflammation and Insulin Sensitivity in Streptozotocin-Nicotinamide-Induced Diabetic Rats

    DOI: 10.3390/cells10102688

    PMID: 34685668

    Sample: plasma
  19. Frontiers in Plant Science (2022) IF: 6.627
    Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

    DOI: 10.3389/fpls.2022.941388

    PMID: 35909740

    Sample: roots or shoots
  20. Nutrients (2022) IF: 6.706
    UPLC-MS/MS Analysis of Naturally Derived Apis mellifera Products and Their Promising Effects against Cadmium-Induced Adverse Effects in Female Rats

    DOI: 10.3390/nu15010119

    Sample: Ovarian Tissues
  21. Antioxidants (2020) IF: 5.014
    Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects

    DOI: 10.3390/antiox9020110

    PMID: 32012711

    Sample: Plasma
  22. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Antidiabetic effects of Psidium x durbanensis Baijnath & Ramcharun ined. (Myrtaceae) leaf extract on streptozotocin-induced diabetes in rats

    DOI: 10.1016/j.jep.2022.115542

    PMID: 35842177

    Sample: liver
  23. Reproductive Biology and Endocrinology (2022) IF: 5.211
    Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice

    DOI: 10.1186/s12958-022-00949-8

    PMID: 35597951

    Sample: testis tissue
  24. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079

    Sample: livers
  25. Food & Function (2021) IF: 5.396
    Supplementation of amino acids and organic acids prevents the increase in blood pressure induced by high salt in Dahl salt-sensitive rats

    DOI: 10.1039/D1FO03577K

    PMID: 34994761

    Sample: renal
  26. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    Sample: serum
  27. Foods (2022) IF: 5.561
    Freshwater Clam Extract Attenuates Indomethacin-Induced Gastric Damage In Vitro and In Vivo

    DOI: 10.3390/foods12010156

    Sample: gastric mucosa
  28. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Notoginsenoside R1 induces oxidative stress and modulates LPS induced immune microenvironment of nasopharyngeal carcinoma

    DOI: 10.1016/j.intimp.2022.109323

    PMID: 36279666

    Sample: NPC cells
  29. INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
    HC067047 as a potent TRPV4 inhibitor repairs endotoxemia colonic injury

    DOI: 10.1016/j.intimp.2022.109648

    Sample: colon
  30. Frontiers in Oncology (2022) IF: 5.738
    ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC.

    DOI: 10.3389/fonc.2022.929838

    Sample: ACHN cell
  31. Frontiers in Pharmacology (2021) IF: 5.811
    Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade.

    DOI: 10.3389/fphar.2021.742954

  32. Pharmaceuticals (2021) IF: 5.863
    Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male Mice

    DOI: 10.3390/ph15010016

    Sample: serum
  33. Frontiers in Pharmacology (2022) IF: 5.988
    Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.

    DOI: 10.3389/fphar.2022.961011

    Sample: testicular tissue
  34. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495

    Sample: testicular tissue
  35. JOURNAL OF INORGANIC BIOCHEMISTRY (2021) IF: 4.155
    Anti-metastasis and anti-proliferation effect of mitochondria-accumulating ruthenium(II) complexes via redox homeostasis disturbance and energy depletion

    DOI: 10.1016/j.jinorgbio.2021.111380

    PMID: 33578250

    Sample: Cell lysate
  36. Foods (2022) IF: 4.35
    Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

    DOI: 10.3390/foods11111551

    Sample: colon tissue
  37. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

    DOI: 10.1016/j.jep.2021.114664

    PMID: 34555451

    Sample: lung tissue
  38. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    Sample: serum
  39. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells

    DOI: 10.1016/j.jep.2022.115110

    PMID: 35181488

  40. International Journal of Nanomedicine (2018) IF: 4.37
    Tanshinol borneol ester on nanostructured lipid carriers has longer brain and systemic effector retention and better antioxidant activity in vivo

    DOI: 10.2147/IJN.S159789

    PMID: 29695905

    Sample: Tissue homogenate
  41. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019) IF: 4.527
    Chronic neurodegeneration by aflatoxin B1 depends on alterations of brain enzyme activity and immunoexpression of astrocyte in male rats

    DOI: 10.1016/j.ecoenv.2019.109407

    PMID: 31279280

    Sample: Tissue homogenate
  42. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

    DOI: 10.1016/j.biopha.2020.109841

    PMID: 31972360

    Sample: Tissue homogenate
  43. MOLECULES (2022) IF: 4.927
    Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

    DOI: 10.3390/molecules27196470

    PMID: 36235007

    Sample: plasma
  44. Oncology Letters (2023) IF: 3.111
    HIF‑1α inhibits ferroptosis and promotes malignant progression in non‑small cell lung cancer by activating the Hippo‑YAP signalling pathway

    DOI: 10.3892/ol.2023.13676

    Sample: A549 cell
  45. EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115
    Neuroprotective effects of brain‐derived neurotrophic factor against amyloid beta 1‐40‐induced retinal and optic nerve damage

    DOI: 10.1111/ejn.14662

    Sample: Tissue homogenate
  46. NEUROSCIENCE LETTERS (2022) IF: 3.197
    Dexmedetomidine prevents hemorrhagic brain injury by reducing damage induced by ferroptosis in mice

    DOI: 10.1016/j.neulet.2022.136842

    PMID: 35995304

    Sample: brain
  47. Animals (2023) IF: 3.231
    The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin Stress

    DOI: 10.3390/ani13030363

    Sample: Plasma
  48. Animals (2022) IF: 3.231
    Expression of Hormones, Cytokines, and Antioxidants in Heat-Stressed Subfertile Female Dromedaries

    DOI: 10.3390/ani12162125

    PMID: 36009715

    Sample: serum
  49. Biomed Research International (2022) IF: 3.246
    Red Raspberry Extract Decreases Depression-Like Behavior in Rats by Modulating Neuroinflammation and Oxidative Stress

    DOI: 10.1155/2022/9943598

    PMID: 35818443

    Sample: hippocampal tissue
  50. Biomed Research International (2021) IF: 3.411
    Antioxidant Activity Derived from Marine Green-Lipped Mussel Perna canaliculus Extracts in Mice

    DOI: 10.1155/2021/1622270

    PMID: 34409099

    Sample: liver,kidney,cardiac
  51. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    Sample: brain tissue
  52. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021) IF: 3.639
    Effects of almonds on ameliorating salt-induced hypertension in Dahl salt-sensitive rats

    DOI: 10.1002/jsfa.11611

    PMID: 34708426

    Sample: renal cortex
  53. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    Sample: Tissue homogenate
  54. NEUROSCIENCE (2023) IF: 3.708
    HC067047 Ameliorates Sepsis-associated Encephalopathy by Suppressing Endoplasmic Reticulum Stress and Oxidative Stress-Induced Pyroptosis in the Hippocampi of Mice

    DOI: 10.1016/j.neuroscience.2023.02.005

    Sample: hippocampi
  55. PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 3.72
    Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in Arabidopsis

    DOI: 10.1016/j.plaphy.2020.02.024

    PMID: 32097848

    Sample: Plant Tissue
  56. CELLULAR SIGNALLING (2020) IF: 3.968
    Circular RNA circ_DROSHA alleviates the neural damage in a cell model of temporal lobe epilepsy through regulating miR-106b-5p/MEF2C axis

    DOI: 10.1016/j.cellsig.2020.109901

    PMID: 33370579

    Sample: SK-N-SH cell,IMR-32 cell
  57. Biomolecules & Therapeutics (2017) IF: 2.075
    Hepaprotective Effect of Standardized Ecklonia stolonifera Formulation on CCl4-Induced Liver Injury in Sprague-Dawley Rats

    DOI: 10.4062/biomolther.2017.199

    PMID: 29129047

    Sample: Tissue homogenate
  58. JOURNAL OF MOLECULAR HISTOLOGY (2020) IF: 2.531
    Oxidative stress induced pyroptosis leads to osteogenic dysfunction of MG63 cells

    DOI: 10.1007/s10735-020-09874-9

    PMID: 32356234

    Sample: Cell culture supernatant
  59. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway

    DOI: 10.1155/2021/2073296

    Sample: BEAS-2B cell
  60. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Etlingera elatior Flower Aqueous Extract Protects against Oxidative Stress-Induced Nephropathy in a Rat Model of Type 2 Diabetes

    DOI: 10.1155/2022/2814196

    PMID: 35502173

    Sample: plasma
  61. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    Antioxidant and antithrombotic effects of green mussels (Perna canaliculus) in rats

    DOI: 10.1111/jfbc.13865

    PMID: 34263474

    Sample: Liver,kidney
  62. Applied Sciences-Basel (2023) IF: 2.838
    Protective Effect of Ethanolic Extract of Djulis Hull on Indomethacin-Induced Gastric Injury

    DOI: 10.3390/app13010594

    Sample: gastric tissue
  63. Analytical Methods (2022) IF: 2.896
    Evaluate the Effect of nitrate and chloride on Cd(II)-Induced Single Cell Oxidative Stress by Scanning Electrochemical Microscopy

    DOI: 10.1039/D2AY00495J

    Sample: MCF-7 cell
  64. ANNALS OF VASCULAR SURGERY (2020) IF: 1.125
    Which Distant Organ is Most Affected by Lower Extremity Ischemia-Reperfusion?

    DOI: 10.1016/j.avsg.2020.01.008

    PMID: 31927058

    Sample: Tissue homogenate
  65. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    Biochemical and histopathological assessment of liver in a rat model of metabolic syndrome induced by high‐carbohydrate high‐fat diet

    DOI: 10.1111/jfbc.13371

    Sample: Tissue homogenate
  66. Molecular Medicine Reports (2018) IF: 1.922
    Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosis

    DOI: 10.3892/mmr.2017.7954

    Sample: Serum
  67. ENVIRONMENTAL MONITORING AND ASSESSMENT (2019) IF: 1.959
    Biochemical investigation of association of arsenic exposure with risk factors of diabetes mellitus in Pakistani population and its validation in animal model

    DOI: 10.1007/s10661-019-7670-2

    PMID: 31346790

    Sample: Blood,Urine
  68. Journal of Evidence-Based Integrative Medicine (2019)
    Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats:

    DOI: 10.1177/2515690X19843832

    Sample: pancreatic,liver
  69. Toxicology Reports (2022)
    Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

    DOI: 10.1016/j.toxrep.2022.06.005

    Sample: plasma
  70. JOURNAL OF ASIA-PACIFIC ENTOMOLOGY (2019) IF: 0.967
    Bombyx mori used as a fast detection model of liver melanization after a clinical drug – Acetaminophen exposure

    DOI: 10.1016/j.aspen.2019.11.009

    Sample: Plasma,Tissue homogenate
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A

Verified Customer

l**ySubmitted [ Jun 03 2019 ]

  • Application:Biochemical
  • Description:The kit is simple to operate, can detect many samples in a batch, and the detection time is short, the calculation is simple, the experimental results are clear and accurate.
Read More
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Q

錯*********nSubmitted [ Jun 16 2023 ]

Asked: There are instructions on the website that can be used in culture supernatantbut but the instruction manual does not.

Reply

A

adminSubmitted [ Jun 16 2023 ]

Answered: Dear HuieLian,thanks for your support.We will reply you by E-mail.Please check your mailbox.

Q

錯*********nSubmitted [ Jun 15 2023 ]

Asked: There are instructions on the website that can be used in culture supernatantbut but the instruction manual does not.

Reply

A

adminSubmitted [ Jun 15 2023 ]

Answered: Dear HuieLian,thanks for your support.We will reply you by E-mail.

Q

錯*********nSubmitted [ Jun 15 2023 ]

Asked: Can I use E-BC-K030-M kit with NRK-52e cells samples?

Reply

A

adminSubmitted [ Jun 15 2023 ]

Answered: Dear HuieLian,thanks for your support.We will reply you by E-mail.

Q

M*****aSubmitted [ Oct 20 2022 ]

Asked: Do I need to make double wells for the standard curve?

Reply

A

adminSubmitted [ Oct 20 2022 ]

Answered: It is suggested to make multiple wells in the marking curve, which can reduce the error.

Q

M*****aSubmitted [ Sep 20 2022 ]

Asked: Our customer bought E-BC-K030-M assay kit for his fibroblast cells sample. Is there any other alternative homogenized medium include 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, and 1 mM EDTA in Appendix II: Sample preparations (in protocol) that can be use? Perhaps PBS?.

Reply

A

adminSubmitted [ Sep 20 2022 ]

Answered: Yes, it can be replaced with normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Q

N**rSubmitted [ Aug 04 2018 ]

Asked: In the operation step use standerd concentration 20 μmol/L but when calculate result and reagent prepration you are use 40 μmol/L what is correct?

Reply

A

adminSubmitted [ Aug 04 2018 ]

Answered: The standard concentration of E-BC-K030 is 40umol/L.

Q

N**rSubmitted [ Aug 04 2018 ]

Asked: Standard conc.use 40micomol or 20

Reply

A

adminSubmitted [ Aug 04 2018 ]

Answered: The standard concentration of E-BC-K030 is 40 umol/L.

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