Reduced Glutathione (GSH) Colorimetric Assay Kit (E-BC-K030-M)
For research use only.
| Detection Principle | Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly. |
| Synonyms | GSH |
| Sample Type | Serum,Plasma,Cell culture supernatant,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405-414 nm) |
| Research Area | Oxidative Stress , Cuproptosis , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2 μmol/L |
| Detection Range | 2-100 μmol/L |
| Precision | inter-assay CV: 3.2% | intra-assay CV: 1.9% |
| Sample Volume | 0.1 mL |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| Hela cell homogenization (0.999 mgprot/mL) | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Mouse serum | 1 |
| 10% Carrot tissue homogenization | 1 |
| 293T supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
