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Cat.No.:E-BC-K030-M
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Protocols for metabolism assays
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Synonyms | GSH |
Sample type | Serum,plasma,cell culture supernatant,tissue,cells |
Sensitivity | 2 μmol/L |
Detection range | 2-100 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 25 min |
Precision | Average inter-assay CV: 3.200%Average intra-assay CV: 1.900% |
Other instruments required | Micropipettor, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
Significant differences can be observed comparing with P group. (*P<0.05, **P
G G Liou et al found that N-acetyl cysteine can reduce propacetamol-induced hepatotoxicity with low dose, but induce with high dose. Reduced glutathione level of mouse liver tissue was determined using GSH colorimetric assay kit (E-BC-K030-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2-100 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
10% Mouse brain tissue homogenization |
1 |
10% Mouse liver tissue homogenization |
1 |
Hela cell homogenization(0.999 mgprot/mL) |
1 |
Rat serum |
1 |
Rat plasma |
1 |
Mouse serum |
1 |
10% Carrot tissue homogenization |
1 |
293T supernatant |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
DOI: 10.3390/ph15010016 |
|
Sample: Serum |
Sample: Brain |
PMID: 36069237 |
Sample: Colon Tissue |
PMID: 35842177 |
Sample: Liver |
PMID: 35489465 |
Sample: Pig Testicular Cell (St Cell) |
Sample: Pancreatic,Liver |
Sample: Plasma |
Sample: Plasma,Tissue Homogenate |
PMID: 31927058 |
Sample: Tissue Homogenate |
DOI: 10.1111/jfbc.13371 |
|
Sample: Tissue Homogenate |
Sample: Serum |
PMID: 31346790 |
Sample: Blood,Urine |
PMID: 29129047 |
Sample: Tissue Homogenate |
PMID: 32356234 |
Sample: Cell Culture Supernatant |
DOI: 10.1155/2021/2073296 |
|
Sample: Beas-2B Cell |
DOI: 10.1155/2022/2814196 |
|
PMID: 35502173 |
Sample: Plasma |
DOI: 10.1111/jfbc.13865 |
|
PMID: 34263474 |
Sample: Liver,Kidney |
DOI: 10.3390/app13010594 |
|
Sample: Gastric Tissue |
DOI: 10.1039/D2AY00495J |
|
Sample: Mcf-7 Cell |
Sample: A549 Cell |
DOI: 10.1111/ejn.14662 |
|
Sample: Tissue Homogenate |
PMID: 35995304 |
Sample: Brain |
DOI: 10.3390/ani13030363 |
|
Sample: Plasma |
DOI: 10.3390/ani12162125 |
|
PMID: 36009715 |
Sample: Serum |
DOI: 10.1155/2022/9943598 |
|
PMID: 35818443 |
Sample: Hippocampal Tissue |
DOI: 10.1155/2021/1622270 |
|
PMID: 34409099 |
Sample: Liver,Kidney,Cardiac |
PMID: 35635784 |
Sample: Brain Tissue |
DOI: 10.1002/jsfa.11611 |
|
PMID: 34708426 |
Sample: Renal Cortex |
PMID: 32220623 |
Sample: Tissue Homogenate |
Sample: Hippocampi |
PMID: 32097848 |
Sample: Plant Tissue |
PMID: 33370579 |
Sample: Sk-N-Sh Cell,Imr-32 Cell |
Sample: Testicular Tissue |
PMID: 33578250 |
Sample: Cell Lysate |
Sample: Colon Tissue |
PMID: 34555451 |
Sample: Lung Tissue |
PMID: 35443217 |
Sample: Serum |
PMID: 35181488 |
DOI: 10.2147/IJN.S159789 |
|
PMID: 29695905 |
Sample: Tissue Homogenate |
PMID: 31279280 |
Sample: Tissue Homogenate |
PMID: 31972360 |
Sample: Tissue Homogenate |
PMID: 36235007 |
Sample: Plasma |
PMID: 32012711 |
Sample: Plasma |
PMID: 35597951 |
Sample: Testis Tissue |
Sample: Livers |
DOI: 10.1039/D1FO03577K |
|
PMID: 34994761 |
Sample: Renal |
PMID: 36230124 |
Sample: Serum |
Sample: Gastric Mucosa |
PMID: 36279666 |
Sample: Npc Cells |
Sample: Colon |
Sample: Achn Cell |
Sample: Testicular Tissue |
Sample: Heart |
PMID: 35883716 |
Sample: T98G Cell,U373 Cell |
PMID: 33809388 |
Sample: Tissue Homogenate |
PMID: 36280221 |
Sample: Skin Tissues |
PMID: 34891114 |
Sample: Serum |
PMID: 34399203 |
Sample: Heart |
PMID: 34685668 |
Sample: Plasma |
PMID: 35909740 |
Sample: Roots Or Shoots |
DOI: 10.3390/nu15010119 |
|
Sample: Ovarian Tissues |
DOI: 10.1155/2022/3593294 |
|
PMID: 36238648 |
Sample: Hippocampus |
PMID: 36358477 |
Sample: Liver,Serum |
Sample: Pbmcs |
PMID: 36290602 |
Sample: Ovarian Tissue |
PMID: 34571217 |
Sample: Liver Organoids (Los) |
PMID: 35709941 |
Sample: Cal-27 Cell |
PMID: 36037900 |
Sample: T3 Strain |
Sample: Mcf-7 Cells |
Verified Customer
l**ySubmitted [ Jun 03 2019 ]
錯*********nSubmitted [ Jun 16 2023 ]
Asked: There are instructions on the website that can be used in culture supernatantbut but the instruction manual does not.
Reply
adminSubmitted [ Jun 16 2023 ]
Answered: Dear HuieLian,thanks for your support.We will reply you by E-mail.Please check your mailbox.
錯*********nSubmitted [ Jun 15 2023 ]
Asked: There are instructions on the website that can be used in culture supernatantbut but the instruction manual does not.
Reply
adminSubmitted [ Jun 15 2023 ]
Answered: Dear HuieLian,thanks for your support.We will reply you by E-mail.
錯*********nSubmitted [ Jun 15 2023 ]
Asked: Can I use E-BC-K030-M kit with NRK-52e cells samples?
Reply
adminSubmitted [ Jun 15 2023 ]
Answered: Dear HuieLian,thanks for your support.We will reply you by E-mail.
M*****aSubmitted [ Oct 20 2022 ]
Asked: Do I need to make double wells for the standard curve?
Reply
adminSubmitted [ Oct 20 2022 ]
Answered: It is suggested to make multiple wells in the marking curve, which can reduce the error.
M*****aSubmitted [ Sep 20 2022 ]
Asked: Our customer bought E-BC-K030-M assay kit for his fibroblast cells sample. Is there any other alternative homogenized medium include 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, and 1 mM EDTA in Appendix II: Sample preparations (in protocol) that can be use? Perhaps PBS?.
Reply
adminSubmitted [ Sep 20 2022 ]
Answered: Yes, it can be replaced with normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
N**rSubmitted [ Aug 04 2018 ]
Asked: In the operation step use standerd concentration 20 μmol/L but when calculate result and reagent prepration you are use 40 μmol/L what is correct?
Reply
adminSubmitted [ Aug 04 2018 ]
Answered: The standard concentration of E-BC-K030 is 40umol/L.
N**rSubmitted [ Aug 04 2018 ]
Asked: Standard conc.use 40micomol or 20
Reply
adminSubmitted [ Aug 04 2018 ]
Answered: The standard concentration of E-BC-K030 is 40 umol/L.