Click here to view cell apoptosis assay products
×

Current Position:Home>>Kits>>Metabolism Assays>> Reduced Glutathione (GSH) Colorimetric Assay Kit

Reduced Glutathione (GSH) Colorimetric Assay Kit

Citations(49)

  • Cat.No.:E-BC-K030-S

  • Detection instrument: Spectrophotometer(420 nm)

Manual MSDS

To Purchase E-BC-K030-S

Size:
  • 100Assays
  • 50Assays
Price: $150
Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

 Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Reduced glutathione (GSH) can react with dithionitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound which has the maximum absorption peak at 420 nm. The GSH content can be calculated by measuring the OD value at 420 nm.

Performance characteristics

Synonyms GSH
Sample type Serum,plasma,tissue,cells
Sensitivity 0.26 mg GSH/L
Detection range 0.26-122.8 mgGSH/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 2.400%Average intra-assay CV: 1.800%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

The level of GSH was significantly changed (*P<0.01) in D100, D200 and Co2+ groups.

D Wang et al investigates the acute toxicity and oxidative damage caused by cobalt-based framework. Reduced glutathione level of Photobacterium phosphoreum T3 was determined using GSH colorimetric assay kit (E-BC-K030-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of pre-experiment and the detection range ( 0.26-122.8 mg GSH/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

10% Mouse brain tissue homogenization

1

10% Rat liver tissue homogenization

1

Human plasma

1

Rat plasma

1

10% Carrot tissue homogenization

1

293T supernatant

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
    A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin

    DOI: 10.1186/s12951-022-01455-0

    PMID: 35568865

    		 Sample: Lewis cell
  2. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling Pathway

    DOI: 10.1155/2022/3593294

    PMID: 36238648

    		 Sample: hippocampus
  3. Antioxidants (2022) IF: 7.675
    Phenolics and Volatile Compounds of Fennel (Foeniculum vulgare) Seeds and Their Sprouts Prevent Oxidative DNA Damage and Ameliorates CCl4-Induced Hepatotoxicity and Oxidative Stress in Rats

    DOI: 10.3390/antiox11122318
    Sample: serum
  4. Antioxidants (2023) IF: 7.675
    Nephroprotective Effect of Fennel (Foeniculum vulgare) Seeds and Their Sprouts on CCl4-Induced Nephrotoxicity and Oxidative Stress in Rats

    DOI: 10.3390/antiox12020325
    Sample: Serum,kidney tissue
  5. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
    Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

    DOI: 10.1016/j.fct.2022.113061

    PMID: 35489465

    		 Sample: pig testicular cell (ST cell)
  6. Antioxidants (2022) IF: 6.313
    Potential Neurotoxic Effects of Glioblastoma-Derived Exosomes in Primary Cultures of Cerebellar Neurons via Oxidant Stress and Glutathione Depletion

    DOI: 10.3390/antiox11071225

    PMID: 35883716

    		 Sample: T98G cell,U373 cell
  7. Antioxidants (2022) IF: 6.313
    Evaluating the Nutritional and Immune Potentiating Characteristics of Unfermented and Fermented Turmeric Camel Milk in Cyclophosphamide-Induced Immunosuppression in Rats

    DOI: 10.3390/antiox11040792

    PMID: 35453477

    		 Sample: blood
  8. Antioxidants (2022) IF: 6.313
    Antioxidative, Antidiabetic, and Hypolipidemic Properties of Probiotic-Enriched Fermented Camel Milk Combined with Salvia officinalis Leaves Hydroalcoholic Extract in Streptozotocin-Induced Diabetes in Rats

    DOI: 10.3390/antiox11040668

    PMID: 35453353

    		 Sample: serum
  9. Antioxidants (2022) IF: 6.313
    Ameliorative and Antioxidative Potential of Lactobacillus plantarum-Fermented Oat (Avena sativa) and Fermented Oat Supplemented with Sidr Honey against Streptozotocin-Induced Type 2 Diabetes in Rats

    DOI: 10.3390/antiox11061122
    Sample: serum
  10. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
    Carboxymethyl cellulose/sericin-based hydrogels with intrinsic antibacterial, antioxidant, and anti-inflammatory properties promote re-epithelization of diabetic wounds in rats

    DOI: 10.1016/j.ijpharm.2022.122328

    PMID: 36280221

    		 Sample: skin tissues
  11. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation

    DOI: 10.1016/j.biopha.2021.112467

    PMID: 34891114

    		 Sample: serum
  12. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats

    DOI: 10.1016/j.biopha.2021.112017

    PMID: 34399203

    		 Sample: heart
  13. Fermentation-Basel (2022) IF: 5.123
    Antidiabetic and Hypolipidemic Efficiency of Lactobacillus plantarum Fermented Oat (Avena sativa) Extract in Streptozotocin-Induced Diabetes in Rats

    DOI: 10.3390/fermentation8060267
    Sample: serum
  14. CHEMICO-BIOLOGICAL INTERACTIONS (2022) IF: 5.194
    PAR1, a therapeutic target for remote lung injury associated with hind limb ischemia/reperfusion: ERK5/KLF2-dependent lung capillary barrier preservation

    DOI: 10.1016/j.cbi.2022.109809

    PMID: 35031271

    		 Sample: lung
  15. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: livers
  16. PHYTOMEDICINE (2022) IF: 5.34
    Protective role of casuarinin from Melastoma malabathricum against a mouse model of 5-fluorouracil–induced intestinal mucositis: Impact on inflammation and gut microbiota dysbiosis

    DOI: 10.1016/j.phymed.2022.154092
    Sample: jejunum tissue
  17. Food & Function (2021) IF: 5.396
    Supplementation of amino acids and organic acids prevents the increase in blood pressure induced by high salt in Dahl salt-sensitive rats

    DOI: 10.1039/D1FO03577K

    PMID: 34994761

    		 Sample: renal
  18. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: serum
  19. Foods (2022) IF: 5.561
    Freshwater Clam Extract Attenuates Indomethacin-Induced Gastric Damage In Vitro and In Vivo

    DOI: 10.3390/foods12010156
    Sample: gastric mucosa
  20. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Notoginsenoside R1 induces oxidative stress and modulates LPS induced immune microenvironment of nasopharyngeal carcinoma

    DOI: 10.1016/j.intimp.2022.109323

    PMID: 36279666

    		 Sample: NPC cells
  21. Frontiers in Oncology (2022) IF: 5.738
    ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC.

    DOI: 10.3389/fonc.2022.929838
    Sample: ACHN cell
  22. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495
    Sample: testicular tissue
  23. Foods (2022) IF: 4.35
    Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

    DOI: 10.3390/foods11111551
    Sample: colon tissue
  24. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

    DOI: 10.1016/j.jep.2021.114664

    PMID: 34555451

    		 Sample: lung tissue
  25. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    		 Sample: serum
  26. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells

    DOI: 10.1016/j.jep.2022.115110

    PMID: 35181488
  27. Scientific Reports (2022) IF: 4.38
    Saffron extract and crocin exert anti-inflammatory and anti-oxidative effects in a repetitive mild traumatic brain injury mouse model

    DOI: 10.1038/s41598-022-09109-9

    PMID: 35322143

    		 Sample: cortical tissue
  28. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 4.432
    Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS

    DOI: 10.1016/j.ejphar.2022.174797
    Sample: Lewis cells (LLC)
  29. MOLECULAR NEUROBIOLOGY (2020) IF: 4.5
    Tannic Acid Provides Neuroprotective Effects Against Traumatic Brain Injury Through the PGC-1α/Nrf2/HO-1 Pathway

    DOI: 10.1007/s12035-020-01924-3

    PMID: 32399817

    		 Sample: Tissue homogenate
  30. Disease Models & Mechanisms (2020) IF: 4.651
    Nrf2/HO-1 mediates the neuroprotective effects of pramipexole by attenuating oxidative damage and mitochondrial perturbation after traumatic brain injury in rats

    DOI: 10.1242/dmm.045021
    Sample: Tissue homogenate
  31. MOLECULES (2022) IF: 4.927
    Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

    DOI: 10.3390/molecules27196470

    PMID: 36235007

    		 Sample: plasma
  32. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Daurisoline alleviated experimental colitis in vivo and in vitro: Involvement of NF-κB and Wnt/β-Catenin pathway

    DOI: 10.1016/j.intimp.2022.108714

    PMID: 35366641

    		 Sample: RAW 264.7 cell
  33. Animals (2023) IF: 3.231
    The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin Stress

    DOI: 10.3390/ani13030363
    Sample: Plasma
  34. Animals (2022) IF: 3.231
    Expression of Hormones, Cytokines, and Antioxidants in Heat-Stressed Subfertile Female Dromedaries

    DOI: 10.3390/ani12162125

    PMID: 36009715

    		 Sample: serum
  35. Environmental Research Communications (2022) IF: 3.237
    Metal accumulation in muscle and oxidative stress response in the liver of juvenile Oreochromis niloticus from contaminated sediment under a simulation of increasing temperature

    DOI: 10.1088/2515-7620/ac803d
    Sample: liver
  36. Biomed Research International (2022) IF: 3.246
    Red Raspberry Extract Decreases Depression-Like Behavior in Rats by Modulating Neuroinflammation and Oxidative Stress

    DOI: 10.1155/2022/9943598

    PMID: 35818443

    		 Sample: hippocampal tissue
  37. Bioengineered (2021) IF: 3.269
    Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axis

    DOI: 10.1080/21655979.2021.2010369

    PMID: 34903141

    		 Sample: ARPE19 cell
  38. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: brain tissue
  39. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021) IF: 3.639
    Effects of almonds on ameliorating salt-induced hypertension in Dahl salt-sensitive rats

    DOI: 10.1002/jsfa.11611

    PMID: 34708426

    		 Sample: renal cortex
  40. LIFE SCIENCES (2020) IF: 3.647
    Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

    DOI: 10.1016/j.lfs.2020.118876

    PMID: 33310035

    		 Sample: tissue
  41. CELLULAR SIGNALLING (2020) IF: 3.968
    Circular RNA circ_DROSHA alleviates the neural damage in a cell model of temporal lobe epilepsy through regulating miR-106b-5p/MEF2C axis

    DOI: 10.1016/j.cellsig.2020.109901

    PMID: 33370579

    		 Sample: SK-N-SH cell,IMR-32 cell
  42. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway

    DOI: 10.1155/2021/2073296
    Sample: BEAS-2B cell
  43. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603
    Sample: brain
  44. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    Antioxidant and antithrombotic effects of green mussels (Perna canaliculus) in rats

    DOI: 10.1111/jfbc.13865

    PMID: 34263474

    		 Sample: Liver，kidney
  45. MOLECULAR BIOLOGY REPORTS (2023) IF: 2.742
    Glioblastoma cell-derived exosomes induce cell death and oxidative stress in primary cultures of olfactory neurons. Role of redox stress

    DOI: 10.1007/s11033-023-08256-0
    Sample: T98G cell lines,U373 cell lines
  46. ECOTOXICOLOGY (2021) IF: 2.823
    Garlic (Allium sativum) and Fu-ling (Poria cocos) mitigate lead toxicity by improving antioxidant defense mechanisms and chelating ability in the liver of grass carp (Ctenopharyngodon idella)

    DOI: 10.1007/s10646-021-02405-6

    PMID: 33830385

    		 Sample: Tissue homogenate
  47. Applied Sciences-Basel (2023) IF: 2.838
    Protective Effect of Ethanolic Extract of Djulis Hull on Indomethacin-Induced Gastric Injury

    DOI: 10.3390/app13010594
    Sample: gastric tissue
  48. Journal of Evidence-Based Integrative Medicine (2019)
    Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats:

    DOI: 10.1177/2515690X19843832
    Sample: pancreatic,liver
  49. Toxicology Reports (2022)
    Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

    DOI: 10.1016/j.toxrep.2022.06.005
    Sample: plasma
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

錯*********nSubmitted [ Jun 16 2023 ]

Asked: What is the difference between E-BC-K030-M kit and E-BC-K030-S kit ?

Reply

A

adminSubmitted [ Jun 16 2023 ]

Answered: Dear HuieLian,thanks for your support.We will reply you by E-mail.Please check your mailbox.

Q

錯*********nSubmitted [ Jun 15 2023 ]

Asked: What is the difference between E-BC-K030-M kit and E-BC-K030-S kit ?

Reply

A

adminSubmitted [ Jun 15 2023 ]

Answered: Dear HuieLian,thanks for your support.We will reply you by E-mail.

Q

M*****iSubmitted [ Aug 11 2022 ]

Asked: I don\'t have enough sample, can I reduce all the reagents equally?

Reply

A

adminSubmitted [ Aug 11 2022 ]

Answered: 

Q

I**********nSubmitted [ Sep 20 2021 ]

Asked: How to store E-BC-K030-S kit. Once the dry ice packed box is opened, how to store the kit? At what temperature kit should be stored? Once opened, how many months it can be used? Please answer .

Reply

A

adminSubmitted [ Sep 20 2021 ]

Answered: Dear Vasudevan, Thanks for your inquiry. this kit can store at 4℃ for 6 months. but some prepared solution must be used at once, please refer to the reagent preparation in the manual.

Q

A*****iSubmitted [ May 20 2019 ]

Asked: what kind of data that measured by this kit? GSH level or GSH Activity? thank you

Reply

A

adminSubmitted [ May 20 2019 ]

Answered: It tests GSH concentration

... Show All Show Less

People Also Bought

About Us

Company Information

Distributors

Contact us

News

Return and Refund Policy

dhl Fedex
Terms and Conditions | Privacy Policy | Cookie Policy  ©2009-2024Elabscience Bionovation Inc. All Rights Reserved.
鄂ICP备18003807号-3
We use cookies to improve your browsing experience, serve personalized ads or content, and for analytics and metrics about our visitors both on this website and other media. By clicking "Accept All", you consent to our use of cookies. To find out more about the cookies we use, see our Cookie Policy
Accept All Reject All
Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha:
Click to load Captcha