Reduced Glutathione (GSH) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K030-M

      • 96T
      • 48T
      - +
      Price: $150
      Can detect 80 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(405-414 nm)

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      Detection principle

      Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.

      Performance characteristics

      Synonyms GSH
      Sample type Serum,plasma,cell culture supernatant,tissue,cells
      Sensitivity 2 μmol/L
      Detection range 2-100 μmol/L
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 25 min
      Precision Average inter-assay CV: 3.2%Average intra-assay CV: 1.9%
      Other instruments required Micropipettor, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2-100 μmol/L).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum


      Human plasma


      10% Mouse brain tissue homogenization


      10% Mouse liver tissue homogenization


      Hela cell  homogenization0.999 mgprot/mL


      Rat serum


      Rat plasma


      Mouse serum


      10% Carrot tissue homogenization


      293T supernatant


      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


      1. The Hepatoprotective Effect of Moringa Oleifera Leaf Aqueous Extract Against Cadmium-Induced Toxicity on Male Rats
        Journal: Journal of Biochemical Technology(2020)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K022-M E-BC-K025-M E-BC-K030-M E-BC-K031-M 
        Sample type: Serum,Tissue homogenate
        Reactivity: Rat
      2. Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits
        Journal: Journal of International Pharmaceutical Research(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K022-M E-BC-K025-M E-BC-K029-S E-BC-K030-M E-BC-K096-M 
        Sample type: Serum
        Reactivity: Rabbit
      3. The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice
        IF: 0.24
        Journal: Latin American Journal of Pharmacy(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K025-S E-BC-K029-S E-BC-K030-M E-BC-K031-S E-BC-K096-S 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
      4. Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study
        IF: 0.395
        Journal: Indian Journal of Animal Research(2019)
        DOI: 10.18805/ijar.B-1157
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K025-M E-BC-K030-M E-BC-K031-M E-BC-K096-M 
        Sample type: Tissue homogenate
        Reactivity: Mussel
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      • Reviews (1)
      • Q&A (2)

      Verified Customer

      l**ySubmitted [ Jun 03 2019 ]

      • Application:Biochemical
      • Description:The kit is simple to operate, can detect many samples in a batch, and the detection time is short, the calculation is simple, the experimental results are clear and accurate.
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      N**rSubmitted [ Aug 04 2018 ]

      Asked: In the operation step use standerd concentration 20 μmol/L but when calculate result and reagent prepration you are use 40 μmol/L what is correct?



      adminSubmitted [ Aug 04 2018 ]

      Answered: The standard concentration of E-BC-K030 is 40umol/L.


      N**rSubmitted [ Aug 04 2018 ]

      Asked: Standard conc.use 40micomol or 20



      adminSubmitted [ Aug 04 2018 ]

      Answered: The standard concentration of E-BC-K030 is 40 umol/L.

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