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Reduced Glutathione (GSH) Colorimetric Assay Kit

Name: Reduced Glutathione (GSH) Colorimetric Assay Kit
Brand: Elabscience
SKU: E-BC-K030-M
Price: 150 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(4)Rating(5)

Catalog number:E-BC-K030-M

Size:

96T
48T

Qty:

- +

Price: $150

Can detect 80 samples if without duplication

Detection method: Colorimetric method

Detection instrument: Microplate reader(405-414 nm)

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Manual
MSDS

Datasheet
Citations(4)
Reviews(1)
Q&A (2)

Detection principle

Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.

Performance characteristics

Synonyms	GSH
Sample type	Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity	2 μmol/L
Detection range	2-100 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	25 min
Precision	Average inter-assay CV: 3.2%Average intra-assay CV: 1.9%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
10% Mouse brain tissue homogenization	1
10% Mouse liver tissue homogenization	1
Hela cell homogenization（0.999 mgprot/mL）	1
Rat serum	1
Rat plasma	1
Mouse serum	1
10% Carrot tissue homogenization	1
293T supernatant	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Related Products

Cat Number	Product Name
E-BC-K096-M	Glutathione Peroxidase (GSH-Px) Activity Assay Kit
E-BC-K097-M	Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
E-BC-K099-S	Glutathione Reductase (GR) Activity Assay Kit
E-BC-K278-S	Glutathione-S-Transferase (GST) Activity Assay Kit

Citations

The Hepatoprotective Effect of Moringa Oleifera Leaf Aqueous Extract Against Cadmium-Induced Toxicity on Male Rats
Journal: Journal of Biochemical Technology(2020)
DOI: 99999999
PMID: 99999999
Products: E-BC-K022-M E-BC-K025-M E-BC-K030-M E-BC-K031-M
Sample type: Serum,Tissue homogenate
Reactivity: Rat
Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits
Journal: Journal of International Pharmaceutical Research(2019)
DOI: 99999999
PMID: 99999999
Products: E-BC-K020-M E-BC-K022-M E-BC-K025-M E-BC-K029-S E-BC-K030-M E-BC-K096-M
Sample type: Serum
Reactivity: Rabbit
The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice
IF: 0.24
Journal: Latin American Journal of Pharmacy(2019)
DOI: 99999999
PMID: 99999999
Products: E-BC-K020-M E-BC-K025-S E-BC-K029-S E-BC-K030-M E-BC-K031-S E-BC-K096-S
Sample type: Serum,Tissue homogenate
Reactivity: Mouse
Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study
IF: 0.395
Journal: Indian Journal of Animal Research(2019)
DOI: 10.18805/ijar.B-1157
PMID: 99999999
Products: E-BC-K020-M E-BC-K025-M E-BC-K030-M E-BC-K031-M E-BC-K096-M
Sample type: Tissue homogenate
Reactivity: Mussel

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Show all (3)
Reviews (1)
Q&A (2)

Verified Customer

l**ySubmitted [ Jun 03 2019 ]

Application:Biochemical
Description:The kit is simple to operate, can detect many samples in a batch, and the detection time is short, the calculation is simple, the experimental results are clear and accurate.

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N**rSubmitted [ Aug 04 2018 ]

Asked: In the operation step use standerd concentration 20 μmol/L but when calculate result and reagent prepration you are use 40 μmol/L what is correct?

adminSubmitted [ Aug 04 2018 ]

Answered: The standard concentration of E-BC-K030 is 40umol/L.

N**rSubmitted [ Aug 04 2018 ]

Asked: Standard conc.use 40micomol or 20

adminSubmitted [ Aug 04 2018 ]

Answered: The standard concentration of E-BC-K030 is 40 umol/L.

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