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Reduced Glutathione (GSH) Colorimetric Assay Kit

Citations(4)Uniprot : yhyj
  • Cat.No.:E-BC-K030-M

  • Detection instrument: Microplate reader(405-414 nm)

To Purchase E-BC-K030-M

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  • 96T
  • 48T
Price: $150
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Detection principle

Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.

Performance characteristics

Synonyms GSH
Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 2 μmol/L
Detection range 2-100 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 25 min
Precision Average inter-assay CV: 3.2%Average intra-assay CV: 1.9%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

10% Mouse brain tissue homogenization

1

10% Mouse liver tissue homogenization

1

Hela cell  homogenization0.999 mgprot/mL

1

Rat serum

1

Rat plasma

1

Mouse serum

1

10% Carrot tissue homogenization

1

293T supernatant

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Biochemical Technology (2020)
    The Hepatoprotective Effect of Moringa Oleifera Leaf Aqueous Extract Against Cadmium-Induced Toxicity on Male Rats

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  2. Journal of International Pharmaceutical Research (2019)
    Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits

    PMID: 99999999

    Sample: Serum
  3. Latin American Journal of Pharmacy (2019) IF: 0.24
    The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  4. Indian Journal of Animal Research (2019) IF: 0.395
    Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study

    DOI: 10.18805/ijar.B-1157

    PMID: 99999999

    Sample: Tissue homogenate
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Reviews/Q&A

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  • Reviews
  • Q&A

Verified Customer

l**ySubmitted [ Jun 03 2019 ]

  • Application:Biochemical
  • Description:The kit is simple to operate, can detect many samples in a batch, and the detection time is short, the calculation is simple, the experimental results are clear and accurate.
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Q

N**rSubmitted [ Aug 04 2018 ]

Asked: In the operation step use standerd concentration 20 μmol/L but when calculate result and reagent prepration you are use 40 μmol/L what is correct?

Reply

A

adminSubmitted [ Aug 04 2018 ]

Answered: The standard concentration of E-BC-K030 is 40umol/L.

Q

N**rSubmitted [ Aug 04 2018 ]

Asked: Standard conc.use 40micomol or 20

Reply

A

adminSubmitted [ Aug 04 2018 ]

Answered: The standard concentration of E-BC-K030 is 40 umol/L.

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