SARS-CoV-2 Neutralization Antibody ELISA Kit

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      Catalog number:E-EL-E606

      Synonyms:COVID-19 Neutralizing Atibody,COVID-19 NAb,SARS-CoV-2 Neutralizing Atibody, Neutralizing Atibody NAb

      Size:
      • 96T
      • 24T
      • 96T*5
      • 96T*10
      Qty:
      - +
      Price: $576

      Reactivity: Human

      Assay type: Competitive-ELISA

      Assay time: 1.5h

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Intended use

      This ELISA kit applies to the in vitro qualitative determination of Neutralization antibodies against SARS-CoV-2 in human serum or plasma.

       

      Test principle

      This Test kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample.
      The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.


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