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SARS-CoV-2 Neutralization Antibody ELISA Kit

  • Cat.No.:E-EL-E606

  • Reactivity: Human
  • Assay Type: One step Competitive-ELISA
  • Assay time: 1.5h

To Purchase E-EL-E606

Size:
  • 96T
  • 24T
  • 96T*5
  • 96T*10
Price: $576
Qty:

Properties

Format 24T, 96T, 96T*5, 96T*10
Assay time 1.5h
Sample type Human serum or plasma
Sample volume 50 μL
Storage 2-8℃
Interpretation Qualitative
Application This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Neutralization Antibody in serum or plasma.
Reproducibility Both intra-CV and inter-CV are < 15%
Synonyms COVID-19 Neutralizing Antibody,COVID-19 NAb,SARS-CoV-2 Neutralizing Antibody,Neutralizing Antibody,NAb

Test Principle

This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.

Assay Procedures

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