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STAT3 Monoclonal Antibody

Cat:E-AB-22166
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:Rat heart,Mouse heart,Hela
Verified Samples in IHC:Human colon carcinoma,Mouse brain
Dilution

WB 1:1000-2000, IHC 1:100-200

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Recombinant Protein
Abbre STAT3
Synonyms 1110034C02Rik;Acute Phase Response Factor;Acute-phase response factor;ADMIO;APRF;AW109958;DNA binding protein APRF;FLJ20882;HIES;MGC16063;Signal transducer and activator of transcription 3 (acute phase response factor);Signal transducer and activator of transcription 3;STAT 3;Stat3;STAT3
Swissprot
Observed MW 79,86kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Protein A purification
Research Areas Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Developmental Biology; Signal Transduction; Stem Cells
Clone No. 3F4
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214).