Superoxide Dismutase (SOD) Typed Activity Assay Kit (Hydroxylamine Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K022-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $280
      Can detect 50 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(550 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

      Performance characteristics

      Synonyms CuZn-SOD,T-SOD
      Sample type serum,plasma,urine,cells,cell culture supernatant,tissue
      Sensitivity 2.03 U/mL
      Detection range 2.03-155 U/mL
      Precision Average inter-assay CV: 7.2%Average intra-assay CV: 4.0%
      Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl)
      Storage Reagent 4: -20℃, others: 2-8℃
      Valid period 6 months

      Dilution of sample

      The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      The volume added to the reaction (μL)

      Mouse serum

      3-5

      20-30

      Human serum

      1

      30-40

      Rat serum

      3-5

      20-30

      Rat plasma

      1-2

      20-30

      Human hydrothorax

      1

      30-50

      Human urine

      1

      30-50

      10% Mouse liver tissue homogenate

      50-80

      20-30

      10% Mouse brain tissue homogenate

      8-12

      20-30

      10% Mouse kidney tissue homogenate

      10-20

      20-30

      HepG2 cells (3.27 mgprot/mL)

      5-10

      20-30

      Note: The diluent is normal saline (0.9% NaCl).
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      • Q&A (3)
      Q

      s************eSubmitted [ Mar 19 2019 ]

      Asked: can we used these kits for microlab 300. Thank you

      Reply

      A

      adminSubmitted [ Mar 19 2019 ]

      Answered: I'm sorry this kit can't be used for microlab 300.

      Q

      A******aSubmitted [ Jan 30 2019 ]

      Asked: Kit no catalog E-BC-K022 can be used for what species? Rat, mice or human? Thank you

      Reply

      A

      adminSubmitted [ Jan 30 2019 ]

      Answered: Hi Agnestia, Thanks for your question. This kit Cat.# E-BC-K022 is an universal kit and can be used for rat, mice, human and other species. Please feel free to let us know if any question. Thank you.

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      Asked: This kit can be used for what? rat, human, or mice? Thank you

      Reply

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