TBP Monoclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Mouse
- Reactivity: Human;Mouse;Rat
- Applications: WB
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:Hela,Mouse brain,Rat brain |
Dilution |
WB 1:1000-2000 Western Blot Operation Guide |
Clonality | Monoclonal |
Immunogen | Recombinant Protein |
Abbre | TBP/TATA Binding Protein |
Synonyms | GTF2D;GTF2D1;HDL4;SCA17;TATA binding factor;TATA box binding protein;TATA box factor;TATA sequence binding protein;TATA sequence-binding protein;TATA-binding factor;TATA-box factor;TATA-box-binding protein;TBP;TBP;TFIID;Transcription initiation factor TFIID TBP subunit |
Swissprot | |
Observed MW |
35-45kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus. |
Tissue Specificity | Widely expressed, with levels highest in the testis and ovary. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Protein A purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling |
Clone No. | 4H2 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. |