Click here to view cell apoptosis assay products
×

Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit

  • Cat.No.:E-BC-K298-M

  • Detection instrument: Microplate reader(530-540 nm)

To Purchase E-BC-K298-M

Size:
  • 96T
  • 48T
Price: $220
Qty:

Welcome to order from local distributors.

Tech Support

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the OD values at 530-540 nm.

Performance characteristics

Synonyms TBARS
Sample type Serum,plasma,animal tissue
Sensitivity 0.85 μmol/L
Detection range 2.6-100 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 7.1%Average intra-assay CV: 4.3%
Other instruments required Vortex mixer, Micropipettor, Water bath
Other reagents required Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.6-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

10% Mouse brain tissue homogenization

1

Human serum

1

Rat serum

1

10% Rat liver tissue homogenization

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role

    DOI: 10.1016/j.biopha.2020.110075

    PMID: 32179202

    Sample: Serum,Tissue homogenate
  2. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

    DOI: 10.1016/j.biopha.2020.109841

    PMID: 31972360

    Sample: Tissue homogenate
  3. American Journal of Physiology-Renal Physiology (2020) IF: 3.144
    Chloroquine attenuates lithium-induced NDI and proliferation of renal collecting duct cells

    DOI: 10.1152/ajprenal.00478.2019

    PMID: 32249612

    Sample: Urine
  4. Indian Journal of Traditional Knowledge (2018) IF: /
    Effects of different doses of Prunus laurocerasus L. leaf extract on oxidative stress, hyperglycaemia and hyperlipidaemia induced by type I diabetes.

    PMID: 99999999

    Sample: Serum,Tissue homogenate
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: