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Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit

Cat.No.:E-BC-K298-M
Detection instrument: Microplate reader(530-540 nm)

Detection method: Colorimetric method

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Detection principle

TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the OD values at 530-540 nm.

Performance characteristics

Synonyms	TBARS
Sample type	Serum,plasma,animal tissue
Sensitivity	0.85 μmol/L
Detection range	2.6-100 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	80 min
Precision	Average inter-assay CV: 7.100%Average intra-assay CV: 4.300%
Other instruments required	Vortex mixer, Micropipettor, Water bath
Other reagents required	Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

High dose EGCG increased the concentration of TBARS in rat liver. (*P<0.05)

KW Parn et al investigate the anti-hypertension effect of epigallocatechin gallate. Thiobarbituric acid reactants (TBARS) level in rat liver was determined using TBARS colorimetric assay kit (E-BC-K298-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.6-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat serum	1
10% Mouse brain tissue homogenization	1
Human serum	1
Rat serum	1
10% Rat liver tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53
Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role

DOI: 10.1016/j.biopha.2020.110075

PMID: 32179202
Sample: Tissue homogenate
TALANTA (2022) IF: 6.556
A simple aptamer/gold nanoparticle aggregation-based colorimetric assay for oxidized low-density lipoprotein determination

DOI: 10.1016/j.talanta.2022.124199

Sample: artificial serum
Nutrients (2022) IF: 6.706
Safety and Efficacy of Dietary Epigallocatechin Gallate Supplementation in Attenuating Hypertension via Its Modulatory Activities on the Intrarenal Renin–Angiotensin System in Spontaneously Hypertensive Rats

DOI: 10.3390/nu14214605

Sample: liver
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice

DOI: 10.3390/ijms23074015

PMID: 35409375
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

DOI: 10.1016/j.biopha.2020.109841

PMID: 31972360
Sample: Tissue homogenate
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY (2020) IF: 3.191
Chloroquine attenuates lithium-induced NDI and proliferation of renal collecting duct cells

DOI: 10.1152/ajprenal.00478.2019

PMID: 32249612
Sample: Urine
Bioengineered (2022) IF: 3.269
Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells

DOI: 10.1080/21655979.2022.2079256

Sample: KLE cell
Processes (2022) IF: 3.352
Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

DOI: 10.3390/pr10071314

Sample: stomach
LIFE SCIENCES (2020) IF: 3.647
Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

DOI: 10.1016/j.lfs.2020.118876

PMID: 33310035
Sample: tissue
JOURNAL OF MEDICINAL FOOD (2021) IF: 2.786
Zingerone Administered Neonatally Prevents the Subsequent Development of High Dietary Fructose-Induced Fatty Liver in Sprague Dawley Rats

DOI: 10.1089/jmf.2020.0189

PMID: 33512269
Sample: plasma
Food Science & Nutrition (2022) IF: 2.863
Burdock (Arctium lappa L.) leaf flavonoids rich in morin and quercetin 3-O-rhamnoside ameliorate lipopolysaccharide-induced inflammation and oxidative stress in RAW264.7 cells

DOI: 10.1002/fsn3.2875

Sample: RAW264.7 cell

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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Aug 02 2022 ]

Asked: Do I need to prepare the acetic acid by myself?

adminSubmitted [ Aug 02 2022 ]

Answered: Yes, you do.

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