Click here to view cell apoptosis assay products

Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit

  • Cat.No.:E-BC-K298-M

  • Detection instrument: Microplate reader(530-540 nm)

To Purchase E-BC-K298-M

  • 96T
  • 48T
Price: $220

Welcome to order from local distributors.

Tech Support

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the OD values at 530-540 nm.

Performance characteristics

Synonyms TBARS
Sample type Serum,plasma,animal tissue
Sensitivity 0.85 μmol/L
Detection range 2.6-100 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 7.1%Average intra-assay CV: 4.3%
Other instruments required Vortex mixer, Micropipettor, Water bath
Other reagents required Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.6-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Rat serum


10% Mouse brain tissue homogenization


Human serum


Rat serum


10% Rat liver tissue homogenization


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role

    DOI: 10.1016/j.biopha.2020.110075

    PMID: 32179202

    Sample: Serum,Tissue homogenate
  2. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

    DOI: 10.1016/j.biopha.2020.109841

    PMID: 31972360

    Sample: Tissue homogenate
  3. American Journal of Physiology-Renal Physiology (2020) IF: 3.144
    Chloroquine attenuates lithium-induced NDI and proliferation of renal collecting duct cells

    DOI: 10.1152/ajprenal.00478.2019

    PMID: 32249612

    Sample: Urine
  4. Indian Journal of Traditional Knowledge (2018) IF: /
    Effects of different doses of Prunus laurocerasus L. leaf extract on oxidative stress, hyperglycaemia and hyperlipidaemia induced by type I diabetes.

    PMID: 99999999

    Sample: Serum,Tissue homogenate
Show AllShorten


  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*When will you use it?