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Thiobarbituric Acid Reactants (TBARS) Fluorometric Assay Kit

Cat.No.:E-BC-K298-F
Detection instrument: Fluorescent Microplate reader(λex/em = 520 nm/550 nm)

Detection method: Fluorescence method

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Detection principle

TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the fluorescence values at the excitation wavelength of 530 nm and the emission wavelength of 550 nm.

Performance characteristics

Synonyms	TBARS
Sample type	Serum,plasma,animal tissue,cells
Sensitivity	0.09 μmol/L
Detection range	0.09-10 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	80 min
Precision	Average inter-assay CV: 2.800%Average intra-assay CV: 1.700%
Other instruments required	Vortex mixer, Water bath, Micropipettor
Other reagents required	Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat serum	1
10% Mouse brain tissue homogenization	1
Human plasma	1
Mouse serum	1
10% Rat liver tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

TALANTA (2022) IF: 6.556
A simple aptamer/gold nanoparticle aggregation-based colorimetric assay for oxidized low-density lipoprotein determination

DOI: 10.1016/j.talanta.2022.124199

Sample: artificial serum
Bioengineered (2022) IF: 3.269
Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells

DOI: 10.1080/21655979.2022.2079256

Sample: KLE cell
Processes (2022) IF: 3.352
Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

DOI: 10.3390/pr10071314

Sample: stomach
Italian Journal of Animal Science (2022) IF: 2.217
Mammary gene expressions and oxidative indicators in ruminal fluid, blood, milk, and mammary tissue of dairy goats fed a total mixed ration containing piper meal (Piper betle L.)

DOI: 10.1080/1828051X.2021.2007173

Sample: Plasma,Milk,Ruminal fluid,Mammary Tissue
Food Science & Nutrition (2022) IF: 2.863
Burdock (Arctium lappa L.) leaf flavonoids rich in morin and quercetin 3-O-rhamnoside ameliorate lipopolysaccharide-induced inflammation and oxidative stress in RAW264.7 cells

DOI: 10.1002/fsn3.2875

Sample: RAW264.7 cell

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