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Thiobarbituric Acid Reactants (TBARS) Fluorometric Assay Kit

  • Cat.No.:E-BC-K298-F

  • Detection instrument: Fluorescent Microplate reader(λex/em = 520 nm/550 nm)

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  • 96T
  • 48T
Price: $240
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Detection principle

TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the fluorescence values at the excitation wavelength of 530 nm and the emission wavelength of 550 nm.

Performance characteristics

Synonyms TBARS
Sample type Serum,plasma,animal tissue,cells
Sensitivity 0.09 μmol/L
Detection range 0.09-10 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 2.8%Average intra-assay CV: 1.7%
Other instruments required Vortex mixer, Water bath, Micropipettor
Other reagents required Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

10% Mouse brain tissue homogenization

1

Human plasma

1

Mouse serum

1

10% Rat liver tissue homogenization

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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