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Thiobarbituric Acid Reactants (TBARS) Fluorometric Assay Kit

  • Cat.No.:E-BC-K298-F

  • Detection instrument: Fluorescent Microplate reader(λex/em = 520 nm/550 nm)

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Price: $240

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Detection principle

TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the fluorescence values at the excitation wavelength of 530 nm and the emission wavelength of 550 nm.

Performance characteristics

Synonyms TBARS
Sample type Serum,plasma,animal tissue,cells
Sensitivity 0.09 μmol/L
Detection range 0.09-10 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 2.8%Average intra-assay CV: 1.7%
Other instruments required Vortex mixer, Water bath, Micropipettor
Other reagents required Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Rat serum


10% Mouse brain tissue homogenization


Human plasma


Mouse serum


10% Rat liver tissue homogenization


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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