Thioredoxin Reductase (TrxR) Activity Assay Kit (E-BC-K548-M)
For research use only.
| Detection Principle |
Thioredoxin system, as one of the most important antioxidant systems in the body, can stabilize the redox balance of organisms, regulate signal transduction. It is also related to cell REDOX reaction, nucleic acid metabolism, cell growth and tumorigenesis. Thioredoxin reductase (TrxR) is a NADPH-dependent dimer selenase containing the flavin adenine dinucleotide (FAD) domain and is a member of the pyridine nucleotide-disulfide oxidoreductase family. The thioredoxin system consists of NADPH, thioredoxin reductase and thioredoxin. The TrxR catalytic substrate makes the reducing agent reduce the color developer with a characteristic absorption peak at 412 nm. |
| Synonyms | TrxR |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (412 nm) |
| Research Area | Oxidative Stress |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.82 U/L |
| Detection Range | 0.82-46.81 U/L |
| Precision | inter-assay CV: 3.2-5.3% | intra-assay CV: 1.5-2.9% |
| Sample Volume | 20 μL(tissue/cell homogenate) |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 2-3 |
| 10% Mouse kidney tissue homogenate | 2-3 |
| 10% Mouse lung tissue homogenate | 2-3 |
| HL-60 cells | 1 |
| CHO cells | 1 |
| Hela cells | 1 |
| Jurkat cells | 1 |
| Molt-4 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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