TNM-FH medium is an improved Grace's insect medium. After proper supplementation, it can be used for the culture of various Lepidoptera insect cells. TNM-FH medium contains amino acids, vitamins, inorganic salts and other ingredients required for various types of cell culture, but does not contain protein, lipids or any growth factors, so this product needs to be used with serum or serum-free additives.

General Information

Product Form	Liquid
Concentration	1×
pH	7.2-7.4
D-Glucose	700mg/L
HEPES	Negative
L-glutamine	600mg/L
NaHCO₃	350mg/L
Phenol red	Negative
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	24 months

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Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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