Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K136-S

      Size:
      • 50 Assays
      • 100 Assays
      Qty:
      - +
      Price: $150

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      General information

      Detection significance

      There are two kinds of antioxidant system, one is enzyme antioxidant system, including superoxide dismutase (SOD),) catalase (CAT), glutathione peroxidase (GSH-Px). The other is non-enzymatic antioxidant systems, including uric acid, vitamin C, vitamin E, glutathione, bilirubin, α-lipoic acid, carotenoid. Antioxidant capacity is thought to be the cumulative effect of all antioxidants in blood and body fluids.

      Detection principle

      A variety of antioxidant macromolecules, antioxidant molecules and enzymes in a system can eliminate all kinds of reactive oxygen species and prevent oxidative stress induced by reactive oxygen species. The total level reflect the total antioxidant capacity in the system. Many antioxidants in the body can reduce Fe3+ to Fe2+ and Fe2+ can form stable complexes with phenanthroline substance. The antioxidant capacity (T-AOC) can be calculated by measuring the absorbance at 520 nm.

      Operation procedures

      Operation steps

      For serum (plasma) and other liquid samples

      (1)   Sample tube: Add 1.0 mL of reagent 1 to 5 mL EP tube.

              Control tube: Add 1.0 mL of reagent 1 to 5 mL EP tube.

      (2)   Sample tube: Add A* mL of sample the tube.

              Control tube: Add nothing.

      (3)   Add 2.0 mL of reagent 2 working solution and 0.5 mL of reagent 3 working solution to sample tube and control tube.

      (4)   Mix fully and incubate the tubes at 37℃ for 30 min.

      (5)   Add 0.1 mL of reagent 5 to sample tube and control tube.

      (6)   Sample tube: Add nothing.

             Control tube: Add A* mL of sample the tube.

      (7)   Mix fully and stand for 10 min at room temperature. Set to zero with double-distilled water and measure the OD value of each tube at 520 nm with 1 cm optical path quartz cuvette.

       

      For tissue and cells samples

      (1)   Sample tube: Add 1.0 mL of reagent 1 to 5 mL EP tube.

             Control tube: Add 1.0 mL of reagent 1 to 5 mL EP tube.

      (2)   Sample tube: Add A* mL of sample the tube.

             Control tube: Add nothing.

      (3)   Add 2.0 mL of reagent 2 working solution and 0.5 mL of reagent 3 working solution to sample tube and control tube.

      (4)   Mix fully and incubate the tubes at 37℃ for 30 min.

      (5)   Add 0.2 mL of reagent 5 to sample tube and control tube.

      (6)   Sample tube: Add nothing.

             Control tube: Add A* mL of sample the tube.

      (7)   Add 0.2 mL of reagent 6 to sample tube and control tube.

      (8)   Mix fully and stand for 10 min at room temperature. Set to zero with double-distilled water and measure the OD value of each tube at 520 nm with 1 cm optical path quartz cuvette.

      Operation table

      For serum (plasma) and other liquid samples


       

      Sample tube

      Control tube

      Reagent 1 (mL)

      1.0

      1.0

      Sample (mL)

      A*

       

      Reagent 2 working solution (mL)

      2.0

      2.0

      Reagent 3 working solution (mL)

      0.5

      0.5

      Mix fully and incubate the tubes at 37 for 30 min.

      Reagent 5 (mL)

      0.1

      0.1

      Sample (mL)

       

      A*

      Mix fully and stand for 10 min at room temperature. Set to zero with double-distilled water and measure the OD value of each tube at 520 nm with 1 cm optical path quartz cuvette.


      For tissue and cells samples

       

      Sample tube

      Control tube

      Reagent 1 (mL)

      1.0

      1.0

      Sample (mL)

      A*

       

      Reagent 2 working solution (mL)

      2.0

      2.0

      Reagent 3 working solution (mL)

      0.5

      0.5

      Mix fully and incubate the tubes at 37 for 30 min.

      Reagent 5 (mL)

      0.2

      0.2

      Sample (mL)

       

      A*

      Reagent 6 (mL)

      0.2

      0.2

      Mix fully and stand for 10 min at room temperature. Set to zero with double-distilled water and measure the OD value of each tube at 520 nm with 1 cm optical path quartz cuvette.


      Performance characteristics

      Technical parameter

      Detection range 0.62-145.2 U/mL Average inter-assay CV 8.2%
      Sensitivity 0.62 U/mL Average intra-assay CV 2.7%
      Average recovery rate 105%
      • Show all (3)
      • Reviews (0)
      • Q&A (3)
      Q

      S****tSubmitted [ Mar 08 2019 ]

      Asked: This kit can use invertebrate hemolymph?

      Reply

      A

      adminSubmitted [ Mar 08 2019 ]

      Answered: Dear Serhat, Yes, the kit can use invertebrate hemolymph, if there is other question, please feel free to ask me. Regards, Cathy

      Q

      fSubmitted [ Dec 01 2018 ]

      Asked:  hi is this elisa kit suitable for detection of total antioxidant capacity in small intestine tissue of rat thank you

      Reply

      A

      adminSubmitted [ Dec 01 2018 ]

      Answered: This kit can dectect small intestine tissue sample.

      Q

      F***hSubmitted [ Nov 30 2018 ]

      Asked: Hi I want to ask if I want to measure Total antioxidant capacity in small intestine tissue of rat and number of test I require 75 sample can I use this kit and how many kit I require if I use kit for 100 assay thank you

      Reply

      A

      adminSubmitted [ Nov 30 2018 ]

      Answered: This kit of 100 assays could detect 50 samples with no duplication. So you need 2 kits of 100 assays if you won't test the samples in duplication.

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