Total Carbonyl Colorimetric Assay Kit (E-BC-K171-M)
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For research use only.
| Detection Principle | Carbonyl can react with 2,4-dinitrophenylhydrazine and produce a kind of reddish brown hydrazone compounds, which has a specific absorbance peak at 370 nm. The content of carbonyl can be calculated according to the absorbance value. |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (365-375 nm, optimum wavelength: 370 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.29 μg/mL |
| Detection Range | 1.29-45 μg/mL |
| Precision | inter-assay CV: 5% | intra-assay CV: 1.9% |
| Sample Volume | 24 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Porcine serum | 1-2 |
| Human serum | 1-2 |
| Human plasma | 1-2 |
| 10% Rat kidney tissue homogenate | 3-5 |
| 10% Rat liver tissue homogenate | 3-5 |
| 10% Epipremnum aureum tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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