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Total Carbonyl Colorimetric Assay Kit

Cat.No.:E-BC-K171-M
Detection instrument: Microplate reader(365-375 nm,optimum wavelength: 370 nm)

Detection method: Colorimetric method

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Detection principle

Carbonyl can react with 2,4-dinitrophenylhydrazine and produce a kind of reddish brown hydrazone compounds, which has a specific absorbance peak at 370 nm. The content of carbonyl can be calculated according to the absorbance value.

Performance characteristics

Sample type	Serum,plasma,tissue
Sensitivity	1.29 μg/mL
Detection range	1.29-45 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 5%Average intra-assay CV: 1.900%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.29-45 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Porcine serum	1-2
Human serum	1-2
Human plasma	1-2
10% Rat kidney tissue homogenate	3-5
10% Rat liver tissue homogenate	3-5
10% Epipremnum aureum tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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