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Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit

  • Cat.No.:E-BC-F032

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

To Purchase E-BC-F032

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Price: $400

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Detection principle

Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.

Performance characteristics

Sample type Serum, plasma, animal tissue, cells
Sensitivity 0.12 μmol/L
Detection range 0.12-30 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 7.3%Average intra-assay CV: 1.7%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-30 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Rat serum


Mouse plasma


Rabbit serum


10% Rat liver tissue homogenate


10% Mouse kidney tissue homogenate


10% Rat brain tissue homogenate


10% Rat spleen tissue homogenate


Jukat cells


Note: The diluent is reagent 1.


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