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Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit

  • Cat.No.:E-BC-F032

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

To Purchase E-BC-F032

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  • 96T
  • 48T
Price: $400
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Detection principle

Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.

Performance characteristics

Sample type Serum, plasma, animal tissue, cells
Sensitivity 0.12 μmol/L
Detection range 0.12-30 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 7.3%Average intra-assay CV: 1.7%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-30 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

100-300

Rat serum

100-300

Mouse plasma

100-300

Rabbit serum

100-300

10% Rat liver tissue homogenate

50-150

10% Mouse kidney tissue homogenate

50-200

10% Rat brain tissue homogenate

200-400

10% Rat spleen tissue homogenate

50-200

Jukat cells

20-50

Note: The diluent is reagent 1.


Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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